Recent changes for CSF for Flow Cytometry (Sendaway Test)
Samples must be marked “Urgent”. Flow cytometry is NOT available after 1pm on Fridays or during weekends/afterhours. Testing during these times require approval from a laboratory haematologist PRIOR to sample collection. Otherwise, the sample may not be tested in time (within 2-4 hours) or tested at all. Samples not processed in time is non-viable and non-diagnostic.
Collection tubes: collection into specialised media (RPMI) tube 2 or RPMI tube 3
Collection tubes: collection into specialised media (RPMI are available from the pathology laboratory- ring flow lab on ex 52596)
CSF specimen rapidly degrades upon lumbar puncture. Cells combined within a CSF specimen have a short bench life and rapidly die. Immediate addition of RPMI medium and prompt delivery to the Flow Cytometry laboratory (within 2 hours of collection, ideally 30 mins) minimises cell loss.
If sample cannot be delivered in 30 minutes use ice.
Maximum 10 hrs storage before testing.