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CSF for Flow Cytometry

Alternative Names:Flow Cytometry CSF
Laboratory:Haematology
Test Code:FLW
Medicare rebatable:No
Registration Instructions:

Please collect a CSF tube with 2-3mL RPMI from Central Specimen Reception BEFORE the CSF sample is collected. CSF should be collected directly into this tube (1 part RPMI; 1 part CSF) to improve sample yield. 

ALL CSF FLOW CYTOMETRY REQUESTS MUST ALSO HAVE CYTOLOGY REQUESTED.

Samples must be marked “Urgent”. Flow cytometry is NOT available after 1pm on Fridays or during weekends/afterhours. Testing during these times require approval from a laboratory haematologist PRIOR to sample collection. Otherwise, the sample may not be tested in time (within 2-4 hours) or tested at all. Samples not processed in time is non-viable and non-diagnostic.

Specimen Types:Cerebrospinal Fluid
Container Types:
RPMI MediaTube 3
RPMI Media Tube 2
Adults Volume or Mass:2 mL
Minimum/Paediatric Volume or Mass:2 mL
Collection & Request Instructions:

Collection tubes: collection into specialised media (RPMI) tube 2 or   RPMI tube 3

Collection tubes: collection into specialised media (RPMI tubes are available from the pathology laboratory Central Specimen Reception or ring the lab haem reg on ex 52596)

CSF specimen rapidly degrades upon lumbar puncture. Cells combined within a CSF specimen have a short bench life and rapidly die. Immediate addition of RPMI medium (if not already collected into the RPMI medium) and prompt delivery to the Flow Cytometry laboratory (within 2 hours of collection, ideally 30 mins) minimises cell loss.

ALL CSF FLOW CYTOMETRY REQUESTS MUST ALSO HAVE CYTOLOGY REQUESTED.

 

 

 

Transport Instructions:Room Temperature
Send to lab immediately

If sample cannot be delivered in 30 minutes use ice.

Processing Instructions:Time critical - do not delay handling !
Store at 4'C

Maximum 10 hrs storage before testing.

Assay Frequency:Weekdays

USEFUL LINKS

 

Frozen Section Booking Form