Pathology Handbook

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CSF for Flow Cytometry

Alternative Names:

Flow Cytometry CSF

Laboratory:

Haematology

Test Code:

FLW

Medicare rebatable:

Specimen Types:

Cerebrospinal Fluid

Container Types:

RPMI MediaTube 3
RPMI Media Tube 2

Adults Volume or Mass:

2 mL

Minimum/Paediatric Volume or Mass:

2 mL

Collection & Request Instructions:

Collection tubes: collection into specialised media (RPMI) tube 2 or RPMI tube 3

Collection tubes: collection into specialised media (RPMI tubes are available from the pathology laboratory Central Specimen Reception or ring the lab haem reg on ex 52596)

CSF specimen rapidly degrades upon lumbar puncture. Cells combined within a CSF specimen have a short bench life and rapidly die. Immediate addition of RPMI medium (if not already collected into the RPMI medium) and prompt delivery to the Flow Cytometry laboratory (within 2 hours of collection, ideally 30 mins) minimises cell loss.

ALL CSF FLOW CYTOMETRY REQUESTS MUST ALSO HAVE CYTOLOGY REQUESTED.

Transport Instructions:

Room Temperature
Send to lab immediately

If sample cannot be delivered in 30 minutes use ice.

Assay Frequency:

Weekdays

USEFUL LINKS

Frozen Section Booking Form

Printable Pathology Request Form

Order of Draw - Blood Samples