Pathology Handbook

Volume (Adults)

0.3 mL

Minimum/Paediatric Volume

0.3 mL

Laboratory

Haematology

Immunology

Laboratory

Haematology

Immunology

Assay name

Allergen to Brewer's yeast

Allergen to Brewer

Laboratory

Haematology

Immunology

Laboratory

Haematology

Immunology

Laboratory

Haematology

Immunology

Laboratory

Haematology

Immunology

Laboratory

Haematology

Immunology

Laboratory

Haematology

Immunology

External Laboratory

Douglas Hanly Moir Pathology

Melbourne Pathology

External Transport Instructions

Send at ambient temperature

Send at 4'C with Ice Pack

Laboratory

Haematology

Immunology

Transport Instructions between sites and/or laboratories

Room Temperature
Use Esky from SDMH or CGMC to Alfred

Room Temperature

Registration Instructions

Turnaround test time approximately 4 weeks. 

All specimens from 18/12/2024 to 05/01/2024 are to be frozen and sent to the referral laboratory from 06/01/2024

Turnaround test time approximately 4 weeks. 

Registration Instructions

Turnaround test time approximately 4 weeks. From December 19/12/2022 to 06/01/2023 Specimens are to be frozen and dispatched from the 09/01/2023.

Turnaround test time approximately 4 weeks. 

Laboratory

Haematology

Immunology

Alternative names

Purkinje Cell Antibody, PCA, PCA-1 antibodies, Anti-Yo antibodies, Yo antibodies, Anti-Neuronal Nuclei antibody, Anti-Hu antibodies, Hu antibodies, Anti Ri antibodies, Ri antibodies, Anti CV2 antibodies, CV2 antibodies, Anti Amphiphysin antibodies, Amphiphysin antibodies, Anti-Ma antibodies, Ma antibodies, Anti-Ma1 antibodies, Ma1 antibodies, Anti-Ma2 antibodies, Ma2 antibodies, Anti-PNMA2(Ma2/Ta) Abs, PNMA2 antibodies, Anti SOX1 Abs, SOX1 antibodies, Anti Glia Nuclear antibodies (AGNA), Glia Nuclear antibodies, Paraneoplastic antibodies, GAD 65

Purkinje Cell Antibody, PCA, PCA-1 antibodies, Anti-Yo antibodies, Yo antibodies, Anti-Neuronal Nuclei antibody, Anti-Hu antibodies, Hu antibodies, Anti Ri antibodies, Ri antibodies, Anti CV2 antibodies, CV2 antibodies, Anti Amphiphysin antibodies, Amphiphysin antibodies, Anti-Ma antibodies, Ma antibodies, Anti-Ma1 antibodies, Ma1 antibodies, Anti-Ma2 antibodies, Ma2 antibodies, Anti-PNMA2(Ma2/Ta) Abs, PNMA2 antibodies, Anti SOX1 Abs, SOX1 antibodies, Anti Glia Nuclear antibodies (AGNA), Glia Nuclear antibodies, Paraneoplastic antibodies

Registration Instructions

Pathologist approval not required for non Northern Health request forms

External Transport Instructions

Send-away department: only send the test if it is requested by the Neurology team, otherwise seek approval from Director of Pathology.

Send-away department: only send the test if it is requested by the Neurology team, otherwise seek approval from Director of Pathology.

Pathologist approval not required for non Northern Health request forms

Transport Instructions between sites and/or laboratories

Room Temperature
Use Esky from SDMH or CGMC to Alfred

Room Temperature

Processing Instructions

If there is a dedicated primary tube - handover to Haem after centrifuge

If the tube is to be shared,  centrifuge, make an aliquot of at least 300uL serum and handover to Haem

If the SST is to be shared, manually centrifuge, prepare an aliquot of at least 300uL of serum and deliver to Haematology

Registration Instructions

CSR pls register as FLW and bring sample to flow department.

Flow scientist to allocate appropriate panel (BCF) as per WI072.

Collect Mon-Fri (before 1pm) only (non-public holidays).

Testing must be performed within 24 hours of collection. Performed on patients from 16 years of age and above.  Patients less than 16 years of age testing needs to be referred to RCH.

CSR pls register as FLW and bring sample to flow department.

Flow scientist to allocate appropriate panel (BCF) as per WI072.

Collect Mon-Thurs only for all sites. For Northern Epping site, can collect on Friday (or day before public holidays) until 1pm

Testing must be performed within 24 hours of collection. Performed on patients from 16 years of age and above.  Patients less than 16 years of age testing needs to be referred to RCH.

Volume (Adults)

0.3 mL

Minimum/Paediatric Volume

0.3 mL

External Transport Instructions

Send at 4'C with Ice Pack

Send at ambient temperature

Processing Instructions

If there is a dedicated primary tube - handover to Haem after centrifuge

If the tube is to be shared,  centrifuge, make an aliquot of at least 300uL serum and handover to Haem

If the SST is to be shared, manually centrifuge, prepare an aliquot of at least 300uL of serum and deliver to Haematology.

Request Group

MDU

MEE

External Laboratory

Microbiological Diagnostic Unit

Frequency

Daily

Registration Instructions

If requested for TB - MYB.  Sent to VIDRL

If requested for fungal culture - BCM. Send to microbiology. 

Volume (Adults)

8 mL

4 mL

Minimum/Paediatric Volume

8 mL

4 mL

Preferred Container Type

EDTA 10 mL

EDTA 4 mL

Collection & Request Instructions

Pathologist approval required prior to ordering test. Contact 8405 2596.

Blueprint genetics buccal swab kit may be obtained from pathology.

Laboratory

Haematology

Immunology

Laboratory

Haematology

Immunology

Alternative names

Amyotrophic lateral sclerosis, Frontotemporal dementia

Request Group

SVC

SSW (SVC)

MBS rebatable

No

Yes

Volume (Adults)

10 mL

3 mL

Minimum/Paediatric Volume

1 mL

Preferred Container Type

EDTA 4 mL

EDTA 10 mL

Collection & Request Instructions

This requst must be approved by the haematology registrar prior to sending

Contact the laboratory prior to collection to determine the best day to collect the specimen.  This requst must be approved by the haematology registrar prior to sending

Processing Instructions

Specimen stability 2 days at room temperature.

External Laboratory

Victorian Clinical Genetics Service

Sydney South West Path (SSWPS) - Concord

Request Group

CRG

MPN

Registration Instructions

Requires approval from Laboratory Haematology registrar or consultant. 

Requires approval from Laboratory Haematology registrar or consultant. 

Calreticulin is no longer available as a single gene mutation testing. It is now tested as part of the MPN NGS panel - clinicians should request for MPN NGS instead.

Request Group

Genomics For Life

GFF

External Laboratory

Genomics For Life

External Transport Instructions

Send at ambient temperature

Registration Instructions

Collect Mon to Fri (on non-public holidays only).

Flow scientist to allocate appropriate panel (48T) as per WI072.

Collect Mon-Thurs only for all sites. For Northern Epping site, can collect on Friday (or day before public holidays) until 1pm

Flow scientist to allocate appropriate panel (48T) as per WI072.

Laboratory

Haematology

Immunology

Request Group

CNG (Chlamydia and Gonorrhoea PCR)

STI (Chlamydia and Gonorrhoea PCR)

Registration Instructions

Request on Northern Health patients must be approved by the Director of Pathology prior to testing.       

1 x LH or Salivary Kit

• Karyotype/Chromosomes/G-banded Karyotype = 1 x 6mL LH

• Salivary Kit may be obtained from pathology.

External request does not require approval. 

Approved indications for Northern Health patients:

• Requested by Paediatrician and clinical notes indicate developmental delay
• Pregnancy loss

Otherwise seek approval from the Director of Pathology prior to sending. 

Collection & Request Instructions

1 x LH or Salivary Kit

• Karyotype/Chromosomes/G-banded Karyotype = 1 x 6mL LH

• Salivary Kit may be obtained from pathology.

External Transport Instructions

Request for Northern Health patient must be approved by the director of Pathology prior to sending

External request does not require approval. 

Approved indications for Northern Health patients:

• Requested by Paediatrician and clinical notes indicate developmental delay
• Pregnancy loss

Otherwise seek approval from the Director of Pathology prior to sending. 

Registration Instructions

Discussion with the Medical Microbiologist or Microbiology registrar is required befroe sending.

Laboratory

Haematology

Immunology

Registration Instructions

Aliquot approximately 1.0 ml of serum into a labelled tube and store in the -20^oC CSR freezer.  

Laboratory

Haematology

Immunology

Registration Instructions

Cost: $50.00

Location: Alfred (ALF), Eastern Health (EAH), Austin (AUP), Royal Melbourne Hospital (SMH), Peter MacCallum (PMC), Dorevitch (DVP), Monash (MON), ACL (ACL), Royal Hobart Hospital (RHH)

Billing: Alfred (ALF), Eastern Health (EAH), Austin (AUP), Royal Melbourne Hospital (SMH), Peter MacCallum (PMC), Dorevitch (DVP), Monash (MON), ACL (ACL), Royal Hobart Hospital (RHH)

Doctor: ALF- HS56H, EAH- RB4JY, AUP - KI0AA, SMH-GK4FJ, PMC-CA62J, MON-JD84B, ACL-DD8GT, RHH-AS41H

Cost: $50.00 - Not Medicare rebatable

Location: Alfred (ALF), Eastern Health (EAH), Austin (AUP), Royal Melbourne Hospital (SMH), Peter MacCallum (PMC), Dorevitch (DVP), Monash (MON), ACL (ACL), Royal Hobart Hospital (RHH), Royal Children's Hosptital (RCH)

Billing: Alfred (ALF), Eastern Health (EAH), Austin (AUP), Royal Melbourne Hospital (SMH), Peter MacCallum (PMC), Dorevitch (DVP), Monash (MON), ACL (ACL), Royal Hobart Hospital (RHH), Royal Children's Hospital (RCH)

Doctor: ALF- HS56H, EAH- RB4JY, AUP - KI0AA, SMH-GK4FJ, PMC-CA62J, MON-JD84B, ACL-DD8GT, RHH-AS41H, RCH-HS06F

Request Group

CVD

TNA

Volume (Adults)

6 mL

0.0 mL

Minimum/Paediatric Volume

1 mL

0.0 mL

Registration Instructions

Test no longer available.  Requesting clinician to call the microbiology pathologist to discuss available options.

Preferred Specimen Type

Serum

No specimen

Preferred Container Type

SST

See Detailed Information

Transport Instructions between sites and/or laboratories

Store at 4C

___

Processing Instructions

Centrifuge

External Laboratory

V.I.D.R.L.

Frequency

Weekly

Laboratory

Pathology Service

Molecular

External Laboratory

Pacific Edge Diagnostics

Alternative names

Pharmacogenomic Screen, myDNA, CYP2C19, CYP2D6, 2D6, 2C19, 2C9, CYP2C9, CYP3A4, CYP3A5, CYP1A2, VKORC1, SLO1B1

Pharmacogenomic Screen, myDNA, CYP2D6, 2D6, 2C19, 2C9, CYP2C9, CYP3A4, CYP3A5, CYP1A2, VKORC1, SLO1B1

Preferred Container Type

See Detailed Information

Urine Bottle PLAIN
Sterile Container

Optional Container Type

Plain 70mL pot
Urine Bottle PLAIN

Registration Instructions

Please provide a completed pathology request form (Paper or EMR), including relevant clinical information/patient history.

Preferred Specimen Type

Bronchial Washings
Bronchoalveolar Lavage

Bronchial Washings
Bronchoalveolar Lavage
Smear

Preferred Container Type

See Detailed Information
Slide Cytomailer
Formalin Pot

Sterile Container
Slide Cytomailer
Formalin Pot

Collection & Request Instructions

FNA Specimen Collection Using Cytolyt (LBC) - For Cytology Tests

Please note: CytoLyt solution is not a suitable medium for the collection of specimens for Flow cytometry, Microbiology, and Biochemistry testing. Please check other laboratory collection requirements.

Collection Containers -  FNA Kit (containing slides, slide mailers and Cytolyt Pots) are available from NPV Anatomical Pathology.
• Specimen Pot (CytoLyt) -  label the pot with the Patients Name, DOB and Specimen type/site.
• Slides - Label the frosted end of 2 slides with Patients Name, DOB and Specimen type/site.

1. After the first pass of the specimen has been aspirated, immediately express a small amount of FNA specimen on to one of the microscope slides and smear gently with the other labelled slide. Allow these to air dry.
2. Express the remaining FNA material into the labelled pot containing Cytolyt.
3. Rinse the needle by taking up the CytoLyt solution into the needle and expressing it .This can be performed a number of times to ensure all material from the needle and syringe has been retrieved.
4. Further passes from the same site can be collected with a new needle and aspirated into the original pot, no further slides are required to be prepared.
5. If several different sites are to be sampled, please use a separate FNA kit, containing a pot of CytoLyt for each site/lesion sampled. Making 2 smears for only for the first pass.

6. Place the specimen pot(s) and slide mailers (containing the slides), in abiohazard bag for transport to Anatomical Pathology.

Please contact NPV Anatomical Pathology for CYTOLYT® SOLUTION FOR FNA PROCEDURES FAQS information sheet. email: anatomical.pathology@nh.org.au telephone: 03 9485 9333

FNA Specimen Collection Using Cytolyt (LBC) - For Cytology Tests

Please note: CytoLyt solution is not a suitable medium for the collection of specimens for Flow cytometry, Microbiology, and Biochemistry testing. Please check other laboratory collection requirements.

Collection Containers -  FNA Kit (containing slides, slide mailers and Cytolyt Pots) are available from NPV Anatomical Pathology.
• Specimen Pot (CytoLyt) -  label the pot with the Patients Name, DOB and Specimen type/site.
• Slides - Label the frosted end of 2 slides with Patients Name, DOB and Specimen type/site.

1. After the first pass of the specimen has been aspirated, immediately express a small amount of FNA specimen on to one of the microscope slides and smear gently with the other labelled slide. Allow these to air dry.
2. Express the remaining FNA material into the labelled pot containing Cytolyt.
3. Rinse the needle by taking up the CytoLyt solution into the needle and expressing it .This can be performed a number of times to ensure all material from the needle and syringe has been retrieved.
4. Further passes from the same site can be collected with a new needle and aspirated into the original pot, no further slides are required to be prepared.
5. If several different sites are to be sampled, please use a separate FNA kit, containing a pot of CytoLyt for each site/lesion sampled. Making 2 smears for only for the first pass.

6. Place the specimen pot(s) and slide mailers (containing the slides), in abiohazard bag for transport to Anatomical Pathology.

Please contact NPV Anatomical Pathology for CYTOLYT® SOLUTION FOR FNA PROCEDURES FAQS information sheet.

email: anatomical.pathology@nh.org.au telephone: 03 9485 9333

Preferred Container Type

See Detailed Information

See Under Collection Instructions

Collection & Request Instructions

FNA Specimen Collection Using Cytolyt (LBC) - For Cytology Tests

Please note: CytoLyt solution is not a suitable medium for the collection of specimens for Flow cytometry, Microbiology, and Biochemistry testing. Please check other laboratory collection requirements.

Collection Containers -  FNA Kit (containing slides, slide mailers and Cytolyt Pots) are available from NPV Anatomical Pathology.
• Specimen Pot (CytoLyt) -  label the pot with the Patients Name, DOB and Specimen type/site.
• Slides - Label the frosted end of 2 slides with Patients Name, DOB and Specimen type/site.

1. After the first pass of the specimen has been aspirated, immediately express a small amount of FNA specimen on to one of the microscope slides and smear gently with the other labelled slide. Allow these to air dry.
2. Express the remaining FNA material into the labelled pot containing Cytolyt.
3. Rinse the needle by taking up the CytoLyt solution into the needle and expressing it .This can be performed a number of times to ensure all material from the needle and syringe has been retrieved.
4. Further passes from the same site can be collected with a new needle and aspirated into the original pot, no further slides are required to be prepared.
5. If several different sites are to be sampled, please use a separate FNA kit, containing a pot of CytoLyt for each site/lesion sampled. Making 2 smears for only for the first pass.

6. Place the specimen pot(s) and slide mailers (containing the slides), in abiohazard bag for transport to Anatomical Pathology.

Please contact NPV Anatomical Pathology for CYTOLYT® SOLUTION FOR FNA PROCEDURES FAQS information sheet.

FNA Specimen Collection Using Cytolyt (LBC) - For Cytology Tests

Please note: CytoLyt solution is not a suitable medium for the collection of specimens for Flow cytometry, Microbiology, and Biochemistry testing. Please check other laboratory collection requirements.

Collection Containers -  FNA Kit (containing slides, slide mailers and Cytolyt Pots) are available from NPV Anatomical Pathology.
• Specimen Pot (CytoLyt) -  label the pot with the Patients Name, DOB and Specimen type/site.
• Slides - Label the frosted end of 2 slides with Patients Name, DOB and Specimen type/site.

1. After the first pass of the specimen has been aspirated, immediately express a small amount of FNA specimen on to one of the microscope slides and smear gently with the other labelled slide. Allow these to air dry.
2. Express the remaining FNA material into the labelled pot containing Cytolyt.
3. Rinse the needle by taking up the CytoLyt solution into the needle and expressing it .This can be performed a number of times to ensure all material from the needle and syringe has been retrieved.
4. Further passes from the same site can be collected with a new needle and aspirated into the original pot, no further slides are required to be prepared.
5. If several different sites are to be sampled, please use a separate FNA kit, containing a pot of CytoLyt for each site/lesion sampled. Making 2 smears for only for the first pass.

6. Place the specimen pot(s) and slide mailers (containing the slides), in abiohazard bag for transport to Anatomical Pathology.

Please contact NPV Anatomical Pathology for CYTOLYT® SOLUTION FOR FNA PROCEDURES FAQS information sheet. email: anatomical.pathology@nh.org.au telephone: 03 9485 9333

Preferred Container Type

Plain 70mL pot
Slide Cytomailer

Slide Cytomailer
Plain 70mL pot
Formalin Pot

Registration Instructions

Please provide a completed pathology request form (Paper or EMR), including relevant clinical information/patient history.

Preferred Container Type

Slide

Slide
Slide Cytomailer

Transport Instructions between sites and/or laboratories

Please ensure Cytology specimens are sent in a separate Biohazard bag from any accompanying Histology biopsy specimens (Formalin pots). Formalin vapour affects cytology specimen preservation.

Transport Instructions between sites and/or laboratories

Room Temperature
Use Esky from SDMH or CGMC to Alfred

Room Temperature

MBS rebatable

No

Yes

Processing Instructions

Centrifuge immediately for 15 minutes at 2000-2500 g.

Centrifuge immediately for 10 minutes at 3500rpm.

Request Group

SSW

DTN

Processing Instructions

Specimen stable at 5 days room temperature

Laboratory

Haematology

Immunology

Alternative names

DAT, DCT, Coombes Test

DAT, DCT, Coombs Test

Request Group

DAT

MDC

Request Group

MCD

MDC

Alternative names

MDC

DCT Coombs test

Request Group

DCT / DAT

MCD

Alternative names

DCT, Coombs Test

MDC

Laboratory

Haematology

Immunology

Registration Instructions

Aliquot approximately 1.0 ml of serum into a labelled tube and store in the -20^oC freezer.

Laboratory

Haematology

Immunology

Registration Instructions

Aliquot approximately 1.0 ml of serum into a labelled tube and store in the -20oC freezer.

Request Group

RBW

ERT

MBS rebatable

Yes

No

Collection & Request Instructions

If the sample is not collected at Northern Hospital Epping i.e. collected at other collection sites (Broadmeadows Hospital, Bundoora Centre and Craigieburn Centre), the primary SST tube shall be centrifuged and the sample kept cool with ice blocks at all times. When the SST sample arrives at the laboratory, aliquot and freeze serum at -20 degrees Celsius.

If the sample is not collected at Northern Hospital Epping i.e. collected at other collection sites (Broadmeadows Hospital, Bundoora Centre and Craigieburn Centre), the primary SST tube shall be centrifuged and the sample kept cool with ice blocks at all times. When the SST sample arrives at the laboratory, aliquot and freeze serum at -20 degrees Celsius.

The test is not Medicare rebatable and costs $45 + GST as of 15/1/25

External Laboratory

Sutherland Hospital (SEALS)

Royal Prince of Wales

Laboratory

Haematology

Immunology

Collection & Request Instructions

Patient must have an LDL >=6.5 mmol for testing to be covered by Medicare.

Without Medicare, the test cost $1200 excl GST as at 31/12/2024

External Transport Instructions

For Northern Health patients, seek approval from Director of Pathology before dispatch 

Laboratory

Haematology

Immunology

Registration Instructions

External request does not require approval. 

Approved indications for Northern Health patients:

• Requested by Paediatrician and clinical notes indicate developmental delay
• For pregnancy loss
• Genetic carrier screening for pregnant women

Otherwise seek approval from the Director of Pathology prior to sending. 

External request does not require approval. 

Approved indications for Northern Health patients:

• Requested by Paediatrician and clinical notes indicate developmental delay
• Genetic carrier screening for pregnant women

Otherwise seek approval from the Director of Pathology prior to sending. 

Laboratory

Haematology

Genetics

Registration Instructions

Unless requested by Paediatrician and clinical notes indicate developmental delay, outpatient request for Northern Health patients must be approved by the Director of Pathology prior to sending. 

Test can also be performed on Salivary Kit (VCGS) available from NPV collection rooms (Northern Hospital)

Private patients: Medicare rebate (bulk billed): $86.15 (PCR test), $172.30 (Southern blot).

Public (Northern Health request form): No charge 

Non-Medicare patients: $101.30 (PCR test), $202.65 (Southern blot).

Reporting time

PCR: 2-3 weeks

Southern blot: 2-3 weeks

External request does not require approval. 

Approved indications for Northern Health patients:

• Requested by Paediatrician and clinical notes indicate developmental delay
• For pregnancy loss
• Genetic carrier screening for pregnant women

Otherwise seek approval from the Director of Pathology prior to sending. 

Collection & Request Instructions

EDTA Blood: Adults/Children: 5 ml

Infants: 1-2 ml

Neonates: 0.5-1 ml

Test can also be performed on Salivary Kit (VCGS) available from NPV collection rooms (Northern Hospital)

Private patients: Medicare rebate (bulk billed): $86.15 (PCR test), $172.30 (Southern blot).

Public (Northern Health request form): No charge 

Non-Medicare patients: $101.30 (PCR test), $202.65 (Southern blot).

Reporting time: PCR: 2-3 weeks, Southern blot: 2-3 weeks

EDTA Blood: Adults/Children: 5 ml; Infants: 1-2 ml; Neonates: 0.5-1 ml

Request Group

RCH

IAI

Alternative names

Glutamic Acid Decarboxylase Antibodies

Glutamic Acid Decarboxylase Antibodies, GAD 65

Laboratory

Haematology

Immunology

Laboratory

Haematology

Immunology

Registration Instructions

Part of the Coeliac Screen.

Part of the Coeliac Screen.

Aliquot approximately 1.0 ml of serum into a labelled tube and store in the -20^oC CSR freezer.   If Transglutaminase Antibodies (TTG) is also requested, it can be shared with GLA.

Laboratory

Haematology

Immunology

Registration Instructions

If a patient vomits within one hour of post glucose load register the fasting glucose sample with routine test code FGF. 

If a patient vomits within one hour of post glucose load register the fasting glucose sample with routine test code FGF. 

If a patient vomits post 60 minute mark continue with the test and register GTT.

MBS rebatable

Yes

No

Registration Instructions

This test is no longer offered at Austin Pathology. Contact Haematology registrar if required.

External Laboratory

Austin Pathology

External Transport Instructions

Send Frozen on Dry Ice

Registration Instructions

Unless indicated otherwise on the request form, also register for Hepatitis A IgM (HAM)

Unless indicated otherwise on the request form, also register for Hepatitis A IgM (HAM). 

Minimum volume 0.5ml.

Registration Instructions

Unless indicated otherwise on the request form, also register for Hepatitis A IgG (HAG)

Unless indicated otherwise on the request form, also register for Hepatitis A IgG (HAG)

Minimum serum volume 0.5 ml.

External Transport Instructions

Send at ambient temperature

Send at 4'C with Ice Pack

Optional Container Type

Dry Swab
EDTA 4 mL

EDTA 4 mL

Alternative names

HSV-1 PCR, HSV-2 PCR, CMV PCR (not viral load), VZV PCR, Varicella Zoster PCR, Viral PCR for Herpes viruses, Swab for Herpes PCR, Adenovirus PCR, Enterovirus PCR

Enterovirus PCR

Assay name

Herpes Virus Multiplex PCR (Sendaway Test)

Herpes Virus Multiplex PCR (Plasma) (Sendaway Test)

Preferred Specimen Type

Swab
Cerebrospinal Fluid
Plasma
Urine random

Plasma
Tissue

Preferred Container Type

See Under Collection Instructions
CSF Tube 3
EDTA 10 mL
Viral Transport Medium Swab
Faecal (Brown Top) Container

See Under Collection Instructions
EDTA 10 mL
Plain 70mL pot

Collection & Request Instructions

Tissue specimens not accepted for testing and need to order HSP (VIDRL)

For quantitative CMV PCR order CMV Viral Load.

A separate EDTA blood tube is essential. It cannot be used for other tests. 4 ml EDTA is acceptable if full.

Use a Gene expert (xpert) swab (red top swab). 

Guidelines for swab collection.

A sterile flocked swab is used to vigorously swab the base of the lesion— applying enough pressure to collect epithelial cells without causing bleeding—and collect vesicular fluid. It is important to collect infected epithelial cells from the base of the lesion because they usually contain a significant amount of virus. 
Break off swab into red top swab container.

For investigation of CMV colitis, a colonic biopsy is recommended. For all other enquires, please contact the Microbiology Registrar.

Tissue specimens not accepted for testing and need to order HSP (VIDRL)

For quantitative CMV PCR order CMV Viral Load.

A separate EDTA blood tube is essential. It cannot be used for other tests. 4 ml EDTA is acceptable if full.

For investigation of CMV colitis, a colonic biopsy is recommended. For all other enquires, please contact the Microbiology Registrar.

Processing Instructions

Please note, Adenovirus PCR on Faeces is sent to a different lab - see separate enty in Handbook

Please note, Adenovirus PCR on Faeces panel FPV - see separate enty in Handbook

Frequency

Weekdays

Assay name

Herpes Multiplex PCR

Herpes Multiplex PCR (Swab)

Assay name

Herpes Multiplex / Meningitis viral panel

Herpes Multiplex PCR

Volume (Adults)

3 mL

Minimum/Paediatric Volume

0.5 mL

Frequency

Twice weekly

Weekdays

Preferred Container Type

Viral Transport Medium
CSF Tube 3
Plain 70mL pot

Viral Transport Medium Swab
CSF Tube 3
Plain 70mL pot

Assay name

Meningitis viral panel

Herpes Multiplex / Meningitis viral panel

Preferred Container Type

Viral Transport Medium Swab
CSF Tube 3
Plain 70mL pot

Viral Transport Medium
CSF Tube 3
Plain 70mL pot

Assay name

Histology - Skin for Immunofluorescence (Sendaway Test)

Histology - Skin for Immunofluorescence

MBS rebatable

No

No

Collection & Request Instructions

Anatomical Pathology: Surgical Specimen Collection Guide

Laboratory

Haematology

Immunology

Assay name

HIV-1 PCR (Sendaway Test)

HIV Proviral DNA (Sendaway Test)

Collection & Request Instructions

Department Head authorisation required.

This test is only performed in exceptional circumstances as HIV serology detects both HIV p24 antigen and HIV antibody.  Discussion with Microbiology Unit Head required before this test will be performed.

Department Head authorisation required.

This test is only performed in exceptional circumstances.

HIV serology is the primary method for HIV screening. 

Discussion with Microbiology Unit Head required before this test will be performed.

Minimum volume is 2ml as per VIDRL Handbook.

Processing Instructions

Centrifuge

Centrifuge
Store at 4'C

Laboratory

Haematology

Immunology

Collection & Request Instructions

HLA typing alone only requires ACD tubes only.

An additional paper request form is required for this test, obtainable from the Australian Red Cross Blood Service website at http://resources.transfusion.com.au/cdm/ref/collection/p16691coll1/id/48

HLA typing alone only requires ACD tubes only.

An additional paper request form is required for this test, obtainable from the Australian Red Cross LifeBlood website at https://www.lifeblood.com.au/sites/default/files/resource-library/2023-02/VTIS_request_form_v8.pdf 

Request Group

HDG

SSW (HDG)

Processing Instructions

Store at 4'C

Do Not Centrifuge
Store at 4'C

External Laboratory

Victorian Clinical Genetics Service

Sydney South West Path (SSWPS) - Concord

Notes

Please see link below for external laboratory requirements and costs

http://www.vcgs.org.au/pathology/sections/MolecularGenetics/?docid=5a502d68-e019-43ee-9d05-9ee53c530da0

Laboratory

Haematology

Immunology

Alternative names

Lymphocyte function test, T cell function test

Laboratory

Haematology

Immunology

Request Group

SNP

IHB

Laboratory

Haematology

Immunology

Registration Instructions

Sendaway test to the Clinical Cytogenetics Service.

Authorisation: Pathologist authorisation required. Sample selection requires pathologist triage.

Resulting: Assay results are incorporated into a supplementary biopsy report issued by the pathologist.

Sendaway test to the Clinical Cytogenetics Service.

Sample selection requires pathologist triage.

Resulting: Assay results are incorporated into a supplementary biopsy report issued by the pathologist.

Request Group

POC

PCO

Assay name

Cytogenetics - Products of Conception (Sendaway Test)

Karyotype - Products of Conception (Sendaway Test)

Alternative names

Chromosome Analysis – Products of Conception, Foetal Chromosome Analysis, Karyotype Analysis – Postnatal, Histology-Cytogenetics (POC), Cord Karyotype

Chromosome Analysis – Products of Conception, Foetal Chromosome Analysis, Karyotype Analysis Postnatal, Histology-Cytogenetics (POC), Cord Karyotype

Processing Instructions

If a specimen needs to be shared between histology and cytogenetics CSR are to contact the Anatomical Pathologist during working hours. After hours and weekends leave a note for the Anatomical Pathologist on the sendaway bench.

If a specimen needs to be shared between histology and cytogenetics during Anatomical Pathology working hours; CSR are to give specimen to AP where it will be appropriately split. Out of hours, specimen is to be placed in the AP bucket in the fridge to be split the next working day.

Request Group

PCO

POC

Laboratory

Haematology

Immunology

Registration Instructions

Collect Mon-Thurs only for all sites. For Northern Epping site, can collect on Friday (or day before public holidays) until 1pm

Flow scientist to allocate appropriate panel (LPD) as per WI072.

Blood and bone marrow specimens (dedicated EDTA tube) are to be kept at room temperature. 

All other specimens are to be refrigerated at 4^oC; CSF and tissue specimens require RPMI to be added.

Flow scientist to allocate appropriate panel (LPD) as per WI072.

Blood and bone marrow specimens (dedicated EDTA tube) are to be kept at room temperature. 

All other specimens are to be refrigerated at 4^oC; CSF and tissue specimens require RPMI to be added.

Collection & Request Instructions

Please provide relevant clinical history and specify exact type of flow cytometric analysis required.

For peripheral blood sample, an FBE must also be requested. Collect 2xEDTA. 

For CSF sample, please collect a tube with 2-3mL RPMI from Central Specimen Reception BEFORE the CSF sample is collected. CSF should be collected directly into this tube (1 part RPMI; 1 part CSF) to improve sample yield. All CSF flow requests must also have CYTOLOGY requested.

Samples must be marked “Urgent”. Flow cytometry is NOT available after 1pm on Fridays or during weekends/afterhours. Testing during these times require approval from a laboratory haematologist PRIOR to sample collection. Otherwise, the sample may not be tested in time (within 2-4 hours for CSF, up to 24 hours for blood sample) or tested at all. Samples not processed in time is non-viable and non-diagnostic. 

Laboratory haematologist can be contacted on 8405 2596 during normal working hours or via switchboard after hours.

Collect Mon-Thurs only for all sites. For Northern Epping site, can collect on Friday (or day before public holidays) until 1pm.

Please provide relevant clinical history and specify exact type of flow cytometric analysis required.

For peripheral blood sample, an FBE must also be requested. Collect 2xEDTA. 

For CSF sample, please collect a tube with 2-3mL RPMI from Central Specimen Reception BEFORE the CSF sample is collected. CSF should be collected directly into this tube (1 part RPMI; 1 part CSF) to improve sample yield. All CSF flow requests must also have CYTOLOGY requested.

Samples must be marked “Urgent”. Flow cytometry is NOT available after 1pm on Fridays or during weekends/afterhours. Testing during these times require approval from a laboratory haematologist PRIOR to sample collection. Otherwise, the sample may not be tested in time (within 2-4 hours for CSF, up to 24 hours for blood sample) or tested at all. Samples not processed in time is non-viable and non-diagnostic. 

Laboratory haematologist can be contacted on 8405 2596 during normal working hours or via switchboard after hours.

Registration Instructions

Flow scientist to allocate appropriate panel (LPD) as per WI072.

Blood and bone marrow specimens (dedicated EDTA tube) are to be kept at room temperature. 

All other specimens are to be refrigerated at 4^oC; CSF and tissue specimens require RPMI to be added.

Collect Mon-Thurs only for all sites. For Northern Epping site, can collect on Friday (or day before public holidays) until 1pm

Flow scientist to allocate appropriate panel (LPD) as per WI072.

Blood and bone marrow specimens (dedicated EDTA tube) are to be kept at room temperature. 

All other specimens are to be refrigerated at 4^oC; CSF and tissue specimens require RPMI to be added.

Registration Instructions

CSR to register with generic FLW panel and bring specimen to flow.

Flow scientist: If lymphoma/leukaemia panel is required, add LPD panel. If sendaway for external leukaemia panel is required, then add FAS panel and re-route sample and request to Alfred Pathology. (Note: for paediatric leukaemia, add RCH panel and re-route sample and request to Royal Children's Hospital).

Blood and bone marrow specimens (dedicated EDTA tube) are to be kept at room temperature. 

All other specimens are to be refrigerated at 4^oC - CSF and tissue specimens require RPMI to be added.

Collect Mon-Thurs only for all sites. For Northern Epping site, can collect on Friday (or day before public holidays) until 1pm

CSR to register with generic FLW panel and bring specimen to flow.

Flow scientist: If lymphoma/leukaemia panel is required, add LPD panel. If sendaway for external leukaemia panel is required, then add FAS panel and re-route sample and request to Alfred Pathology. (Note: for paediatric leukaemia, add RCH panel and re-route sample and request to Royal Children's Hospital).

Blood and bone marrow specimens (dedicated EDTA tube) are to be kept at room temperature. 

All other specimens are to be refrigerated at 4^oC - CSF and tissue specimens require RPMI to be added.

Collection & Request Instructions

Please collect 2 x 2.7 mL coagulation (blue top) when this is requested by itself.

Can be requested with AT, Protein C, Protein S and coagulation profile. Please collect total 4 x 2.7 mL tubes for this test combination.

Includes APTT Lupus Screen/Lupus Confirm and DRVVT Lupus Screen/Lupus Confirm.

Registration Instructions

Collect Mon to Fri (on non-public holidays only).

Flow scientist to allocate appropriate panel (LYM) as per WI072. For T or B cells alone, use a separate panel code.

Testing must be performed within 24 hours of collection.  External laboratory only performs test during weekdays. Performed on patients from 16 years of age and above.  Patients less than 16 years of age testing needs to be referred to RCH.

Collect Mon-Thurs only for all sites. For Northern Epping site, can collect on Friday (or day before public holidays) until 1pm

Flow scientist to allocate appropriate panel (LYM) as per WI072. For T or B cells alone, use a separate panel code.

Testing must be performed within 24 hours of collection.  External laboratory only performs test during weekdays. Performed on patients from 16 years of age and above.  Patients less than 16 years of age testing needs to be referred to RCH.

Assay name

Lymphoid NGS (Sendaway Test)

TP53 Mutation (Sendaway Test)

Alternative names

LNGS, MS LNGS, Lymphoid Next Generation Sequencing Panel

Tumour Protein 53 Mutation

Request Group

LNG

See registration.

MBS rebatable

Yes

No

Volume (Adults)

20 mL

Registration Instructions

Haematology registrar / Haematologist approval prior to dispatch 

The attached request form must be completed:

https://www.petermac.org/document/molecular-haematology-request-slip-2/viewdocument/992

Haematology registrar / Haematologist approval prior to dispatch

This test is no longer available as a single gene mutation testing - it forms part of the MPN NGS panel at PMCC, haematological malignancy gene panel at PMCC and the myeloid NGS panel at the Alfred. Clinicians to decide which is the most relevant panel. Refer to the relevant test panels.

Processing Instructions

Do Not Centrifuge
Store at Room Temperature

Processing Instructions

Store at 4'C if there is a delay in sending sample.

External Laboratory

Peter MacCallum Cancer Centre (PMCC)

External Transport Instructions

Send at 4'C with Ice Pack

Frequency

Fortnightly

Laboratory

Haematology

Immunology

Laboratory

Haematology

Immunology

Assay name

Memory T cells

Memory T cells (sendaway test)

Assay name

Methylation study for Prada -Willi Syndrome

Methylation study for Prader -Willi Syndrome

Alternative names

Prada -Willi Syndrome, Prada Willi Syndrome

Prader -Willi Syndrome, Prader Willi Syndrome

Processing Instructions

Store at Room Temperature

Store at 4'C

Collection & Request Instructions

Specimen MUST be delivered to laboratory within <48 hours from collection (Monday - Thursday, excluding public holidays). For identification of carriers of inherited venous thrombophilia. General population screening and routine screening before prescribing oral contraceptives or hormone replacement therapy are not recommended. test should only be requested by a practioner skilled in genetic counselling of thrombophilia. The significance of a polymorphism in an affected individual is uncertain. Test is Methyl Tetrahydrofolate Reductase C677T.

 Homozygous mutation may be associated with increased thrombotic risk.

Requires own tube. Specimen MUST be delivered to laboratory within 48 hours from collection (Monday - Thursday, excluding public holidays).

For identification of carriers of inherited venous thrombophilia. General population screening and routine screening before prescribing oral contraceptives or hormone replacement therapy are not recommended. Test should only be requested by a practioner skilled in genetic counselling of thrombophilia. The significance of a polymorphism in an affected individual is uncertain. Test is Methyl Tetrahydrofolate Reductase C667T.

 Homozygous mutation may be associated with increased thrombotic risk.

Processing Instructions

This requst must be approved by the haematology registrar prior to sending.

Requsts on Northern Health patient must be approved by the haematology registrar/haematologist prior to sending.

External Transport Instructions

This requst must be approved by the haematology registrar prior to sending

Requsts on Northern Health patient must be approved by the haematology registrar/haematologist prior to sending.

Request Group

VCS

VCG

Registration Instructions

Director of Pathology approval required for Northern Health outpatient requests.  RPMI not necessary however if added will not affect test.

EDTA or Salivary kit are the required samples. For product of conception (POC) see "Cytogentics - Products of Conception"

Private: $595

Public (Northern Health request form): No charge 

Non-Medicare patients: $510.40 - $589.90

Microarray and Fragile X testing can be performed on one EDTA specimen  

Director of Pathology approval required for Northern Health outpatient requests.  RPMI not necessary however if added will not affect test.

EDTA or Salivary kit are the required samples. For product of conception (POC) see "Cytogentics - Products of Conception"

For external request forms this test is covered by Medicare if eligibility requirements are met.

Private: $595

Public (Northern Health request form): No charge 

Non-Medicare patients: $510.40 - $589.90

Microarray and Fragile X testing can be performed on one EDTA specimen  

Collection & Request Instructions

Adult-child: 5ml EDTA

Infant: 0.5-1.0ml EDTA

Adult-child: 5ml EDTA

Infant: 0.5-1.0ml EDTA

For external request forms this test is covered by Medicare if eligibility requirements are met.

Request Group

MPL

MPN

MBS rebatable

No

Yes

Registration Instructions

Haematology registrar / Haematologist approval prior to dispatch

Haematology registrar / Haematologist approval prior to dispatch

This test is now part of MPN NGS, and is no longer available as a single gene mutation testing.

Registration Instructions

• Muscle Biopsy needs to be received in Northern Pathology lab by 11:00am for transportation to Alfred Pathology.
• NPV staff to notify VNLS when sample is ready for dispatch and discuss transportation arrangements (9076 5448)
• Time Critical Sample: Please Send to VNLS / Anatomical Pathology (ALFRED) Immediately

• Muscle Biopsy needs to be received in Northern Pathology lab by 11:00am for transportation to Alfred Pathology.
• NPV staff to notify VNLS when sample is ready for dispatch and discuss transportation arrangements (9076 5448)
• Time Critical Sample: Please Send to VNLS / Anatomical Pathology (ALFRED) Immediately
• Muscle tissue culture are performed inhouse

Laboratory

Haematology

Immunology

Assay name

Mycoplasma genitalium PCR (STI/STD Screen)

Mycoplasma genitalium PCR (Urine and genital swabs)

Request Group

MYG (SWAB), MYU (URINE)

STI

Preferred Container Type

Plain 70mL pot
Dry Swab

Plain 70mL pot
Viral Transport Medium Swab

Optional Container Type

Viral Transport Medium Swab

External Laboratory

Dorevitch Pathology

External Transport Instructions

Send at 4'C with Ice Pack

Frequency

Weekly

3 x weekly (thrice)

Laboratory

Haematology

Immunology

Laboratory

Haematology

Immunology

Assay name

Myotonic Dystophy Type 2 Exclusion (Sendaway Test)

Myotonic Dystophy Type 2 CNBP gene (Sendaway Test)

Alternative names

DM2 Exclusion

DM2 CNBP

Laboratory

Immunology

Genetics

External Transport Instructions

This requst must be approved by the haematology registrar prior to sending

For Northern Health patient, unless requested by Neurologist, this requst must be approved by the Director of Pathology prior to sending. 

Request Group

MYD

SSW (MYD)

Preferred Container Type

EDTA 4 mL

EDTA 10 mL

External Laboratory

PathWest QEII Medical Centre

Sydney South West Path (SSWPS) - Concord

Laboratory

Haematology

Immunology

Processing Instructions

Do Not Centrifuge
Store at 4'C

Do Not Centrifuge
Store at Room Temperature

Request Group

MD1

SSW (MD1)

Volume (Adults)

10 mL

4 mL

Minimum/Paediatric Volume

5 mL

4 mL

External Laboratory

Victorian Clinical Genetics Service

Sydney South West Path (SSWPS) - Concord

External Transport Instructions

Send at 4'C with Ice Pack

Send at ambient temperature

External Transport Instructions

For Northern Health patients, if not requested by Neurology, please seek approval from Director of Pathology prior to sending

For Northern Health patients, if not requested by Neurology, please seek approval from Director of Pathology prior to sending

Frequency

Monthly

Notes

Please see link below for external laboratory requirements and costs

https://vcgs.org.au/tests/myotonic-dystrophy/

Registration Instructions

Test only performed on a Tuesday at the Royal Childrens Hospital. Specimen must arrive at the testing laboratory before 1pm. Do not collect on any other day.

Test only performed on a Tuesday at the Royal Childrens Hospital.Specimen must be collected prior to 8am on a Tuesday at the Epping campus only so that the specimen can arrive at the Royal Childrens Hospital before 1pm. Do not collect on any other day. 

Collection & Request Instructions

Test only performed on a Tuesday at the Royal Childrens Hospital. Specimen must arrive at the testing laboratory before 1pm. Do not collect on any other day.

Test only performed on a Tuesday at the Royal Childrens Hospital. Specimen must be collected prior to 8am on a Tuesday at the Epping campus only so that the specimen can arrive at the Royal Childrens Hospital before 1pm. Do not collect on any other day.

Collection & Request Instructions

Test only performed on a Tuesday at the Royal Childrens Hospital. Specimen must arrive at the testing laboratory before 1pm. Do not collect on any other day.

Preferred Container Type

ACD
EDTA

ACD and EDTA

Preferred Container Type

Lithium Heparin (without Gel)

ACD
EDTA

Preferred Container Type

Slide

Slide
Slide Cytomailer

Transport Instructions between sites and/or laboratories

Please ensure Cytology specimens are sent in a separate Biohazard bag from any accompanying Histology biopsy specimens (Formalin pots). Formalin vapour affects cytology specimen preservation.

Alternative names

Paranodal antibodies, NF-155/186, Contactin-1, CASPR-1

Laboratory

Pathology Service

Immunology

Request Group

FFL

SSW

Processing Instructions

Centrifuge
Aliquot
Store at 4'C

Centrifuge
Aliquot
Store frozen

External Laboratory

Forefront

Sydney South West Path (SSWPS) - RPA

External Transport Instructions

Send at 4'C with Ice Pack

Send Frozen on Dry Ice

Registration Instructions

Request Form only required. Forward immediately to Blood Bank.

Request Form only required. Forward immediately to Blood Bank.

Approval is required from one of the following Northern Hospital Senior Clinical Staff members: Director of Anaesthesia, Director of ICU or Consultant Haematologist. Refer to Prompt (Blood- Administration of Clotting Factors) for further information.

Registration Instructions

Doctor : NHIC

Bill to : INFOE

Tests included:

HIV Antigen/Antibody Combo

Anti-Hepatitis C Antibody

Hepatitis B Surface Antigen

Can register under the Northern Health UR number.

Doctor : NHIC

Bill to : INFOE

Tests included:

HIV Antigen/Antibody Combo

Anti-Hepatitis C Antibody

Hepatitis B Surface Antigen

Registration Instructions

This is a CSF test. Serum sample must be collected at the same time for interpretation of results. In the instance where a serum is not received an alternate serum that has been collected within 24 hours of the CSF may be used. Serum alone is not suitable for this test. 

The SST tube (OLI) MUST be sent together with the CSF (COB) and never separately. 

This is a CSF test. Serum sample must be collected at the same time for interpretation of results. In the instance where a serum is not received an alternate serum that has been collected within 24 hours of the CSF may be used. Serum alone is not suitable for this test. 

The SST tube (OLI) MUST be sent together with the CSF (COB) and never separately.   Keep the SST tube in the sendaway department until testing has been completed on the CSF specimen.

Laboratory

Haematology

Immunology

Registration Instructions

All PFA-200 requests MUST also have FBE requested. Please register PFA and FBE panels.

This test can only be collected at Northern Pathology Victoria Epping (TNH) collection rooms and performed on Tuesdays and Thursdays (between 9am to 3pm). Outside of these days, please discuss with Laboratory Haematology 8405 8369.

All PFA-200 requests MUST also have FBE requested. Please register PFA and FBE panels.

This test can only be collected at Northern Pathology Victoria Epping (TNH) collection rooms and performed on Tuesdays and Thursdays (between 9am to 3pm) Excluding Public Holidays. Outside of these days, please discuss with Laboratory Haematology 8405 8369.

Collection & Request Instructions

Patients should be provided with the patient information sheet. Collectors, please record patient medication on request slip or the collection staff checklist.

This test can only be collected at Northern Pathology Victoria Epping (TNH) collection rooms and performed on Tuesdays and Thursdays (between 9am to 3pm). Outside of these days, please discuss with Laboratory Haematology 8405 8369.

Patients should be provided with the patient information sheet. Collectors, please record patient medication on request slip or the collection staff checklist.

This test can only be collected at Northern Pathology Victoria Epping (TNH) collection rooms and performed on Tuesdays and Thursdays (between 9am to 3pm) Excluding Public Holidays. Outside of these days, please discuss with Laboratory Haematology 8405 8369.

Laboratory

Haematology

Immunology

MBS rebatable

Yes

No

Registration Instructions

Non-CMBS test - $88. Discuss with Laboratory Haematology Registrar/Consultant.

Update 11/11/2024: Please note that PAI testing is not currently being offered at Monash Health Pathology. Please contact Special Coagulation lab at Monash Pathology on 9594 6751 for more information.

Preferred Container Type

EDTA 6 mL
EDTA Microtainer

Report Only

Laboratory

Clinical Biochemistry

Pathology Service

Laboratory

Haematology

Immunology

Assay name

Prothrombinex Issue

Prothrombin Complex Issue

Alternative names

Prothrombinex Release, PTX Issue

Prothrombinex Release, PTX Issue, Beriplex Resease

Registration Instructions

Request Form only required. Forward immediately to Blood Bank.

Request Form only required. Forward immediately to Blood Bank.

Prothrombinex will be transitioned to Beriplex on Monday 25 November 2024. Refer to Prompt protocols for further details (Blood - Administration of Clotting Factors, Haematology - Thrombosis & Haemostasis)

External Transport Instructions

Send at ambient temperature

Optional Container Type

Lithium Heparin (without Gel)

Collection & Request Instructions

Available only by arrangement via The Children Hospital at Westmead Haematologist via switchboard (02) 9845 5000

Transport Instructions between sites and/or laboratories

Room Temperature

Store at 4C

Transport Instructions between sites and/or laboratories

Specimen stability from collection to processing: 24 hours refrigerated

Must arrive in Westmead lab within 24 hours of collection. Transport refrigerated

External Laboratory

Royal Children's Hospital Laboratory Services

The Children's Hospital at Westmead

External Transport Instructions

The Children's Hospital at Westmead Pathology Specimen Reception
Corner Hawkesbury Road & Hainsworth Street
Westmead NSW 2145
AUS

Ph:(02) 7825 1833

Fax:(02) 7825 3332

Frequency

By Arrangement

Laboratory

Haematology

Immunology

Laboratory

Haematology

Immunology

Laboratory

Haematology

Immunology

Processing Instructions

Centrifuge
Aliquot
Store at -70'C

Centrifuge
Aliquot
Store at -80'C

Collection & Request Instructions

Test cost $60

FREEZE the serum/plasma within 24 hours of collection and send TWO (2) serum aliquots (min 100uL) to RCH FROZEN.

Test cost $70

FREEZE the serum/plasma within 24 hours of collection and send TWO (2) serum aliquots (min 100uL) to RCH FROZEN.

Processing Instructions

Immediate spin, separate serum or plasma. Freeze 3 aliquots (min 100uL) and freeze at -70C freexer

Immediate spin, separate serum or plasma. Freeze TWO aliquots (min 100uL) and freeze at -80C freezer

Request Group

SSW

SCE

Assay name

TB culture (sputum)

TB culture (respiratory specimens)

Request Group

TCO

TBC

MBS rebatable

No

Yes

Preferred Specimen Type

Sputum

Sputum
Bronchoalveolar Lavage
Bronchial Washings

Laboratory

Blood Bank

Haematology

Registration Instructions

2x EDTA lavender top tube required for thalassaemia testing (1x Hb Electophoresis, 1x DNA testing if required)

1x Serum gold top tube

Please register THL, FBE and FER panel when for all thalassaemia screens.

DO NOT SEND TO MONASH DIRECTLY FROM CSR. If the word gene or DNA is used in the request, this is for genetic test. Thalassaemia genetic test is performed at Monash, register as MON. ALL Thalassaemia DNA analysis must be approved by the Haematology Scientist, Registrar or Consultant first. DO NOT SEND TO MONASH DIRECTLY FROM CSR. All samples requiring DNA analysis will be packaged up by the scientist prior to handing over to the sendaway department for sendout. 

FBE, ferritin and Hb electrophoresis results must be sent together with second EDTA tube.

Patient needs to provided Australian Medicare number and Victoria address for state goverment grant (No charge). For Overseas residents with Victorian address a bill will be issued.

1x EDTA lavender top tube required for thalassaemia testing (Hb Electophoresis and DNA testing if required)

1x Serum gold top tube

Please register THL, FBE and FER panel when for all thalassaemia screens.

DO NOT SEND TO MONASH DIRECTLY FROM CSR. If the word gene or DNA is used in the request, this is for genetic test. Thalassaemia genetic test is performed at Monash, register as MON. ALL Thalassaemia DNA analysis must be approved by the Haematology Scientist, Registrar or Consultant first. DO NOT SEND TO MONASH DIRECTLY FROM CSR. All samples requiring DNA analysis will be packaged up by the scientist prior to handing over to the sendaway department for sendout. 

FBE, ferritin and Hb electrophoresis results must be sent together with second EDTA tube.

Patient needs to provided Australian Medicare number and Victoria address for state goverment grant (No charge). For Overseas residents with Victorian address a bill will be issued.

MBS rebatable

Yes

No

Registration Instructions

Non-CMBS test - $121. Discuss with Laboratory Registrar/Consultant prior to sending out.

Laboratory

Haematology

Immunology

Request Group

TP5

See registration.

Registration Instructions

Haematology registrar / Haematologist approval prior to dispatch

Refer to https://www.austinpathology.org.au/test-directory/1781 for molecular pathology request.

Haematology registrar / Haematologist approval prior to dispatch

This test is no longer available as a single gene mutation testing - it forms part of the MPN NGS panel at PMCC, haematological malignancy gene panel at PMCC and the myeloid NGS panel at the Alfred. Clinicians to decide which is the most relevant panel. Refer to the relevant test panels.

External Laboratory

Austin Pathology

External Transport Instructions

Send at 4'C with Ice Pack

External Transport Instructions

Only if single gene tp53 is requested. If full pan haem panel is required, this would need to be discussed with the Haematologist/Lab Reg

Laboratory

Haematology

Immunology

Registration Instructions

Part of the Coeliac Screen.

Part of the Coeliac Screen.

Aliquot approximately 1.0 ml of serum into a labelled tube and store in the -20^oC CSR freezer.   If Gliadin Antibody (GLA) is also requested, it can be shared with TTG.

Request Group

TRV

STI (Urine, Vaginal, Urethral, Cervical)

Preferred Container Type

Dry Swab

Viral Transport Medium Swab

Collection & Request Instructions

High vaginal swab and urine only validated specimen sites.

Genital swabs and urine only validated specimen sites.

External Laboratory

Royal Children's Hospital Laboratory Services

External Transport Instructions

Send at 4'C with Ice Pack

External Transport Instructions

Direct external contact number: 9345 4673

Frequency

Twice weekly

3 x weekly (thrice)

Laboratory

Haematology

Immunology

Assay name

Ureaplasma species PCR

Ureaplasma species PCR (Urine and genital swabs)

Request Group

URP (SWAB), URU (URINE)

STI

MBS rebatable

No

Yes

Preferred Specimen Type

Urine Midstream
Swab

Urine First Catch
Swab

Preferred Container Type

Plain 70mL pot
Gel Swab

Plain 70mL pot
Viral Transport Medium Swab

Transport Instructions between sites and/or laboratories

Store at 4C

Room Temperature

External Laboratory

Dorevitch Pathology

External Transport Instructions

Send at 4'C with Ice Pack

Frequency

Weekly

3 x weekly (thrice)

Assay name

Ristocetin Cofactor

von Willebrand factor Antigen

Alternative names

RiCoF, von Willebrand activity, vWF activity

vWF antigen

Request Group

VWR

VWA

Registration Instructions

Use VWA is vWF antigen (single test) only is requested. If von Willebrand studies or von Willebrand factor activity are requested, use VWR, F8.

Collection & Request Instructions

Usually ordered as part of von Willebrand factor (vWF) studies, which include von Willebrand factor antigen (vWF Ag), FVIII and Ristocetin (RicoF).

If no other tests in vWF studies are ordered, contact request doctor to confirm that no other tests are required.

Usually ordered as part of von Willebrand factor (vWF) studies, which include von Willebrand factor antigen (vWF Ag), FVIII, vWF collagen binding assay (CBA) and Ristocetin (RicoF).

If no other tests in vWF studies are ordered, contact request doctor to confirm that no other tests are required.

Processing Instructions

Centrifuge immediately for 15 minutes at 2000-2500 g.

Then aliquot plasma into a separate tube. Centrifuge again for 15 minuts at 2000-2500 g.

Centrifuge immediately.

Assay name

Von Willebrand Factor Antigen

Von Willebrand studies

Alternative names

von Willebrand factor, vWF Ag, von Willebrand factor studies

von Willebrand factor, vWF Ag, von Willebrand factor studies, von Willebrand factor activity, Ristocetin activity, collagen binding assay

Request Group

VWA, VWR, F8

VWR, F8

MBS rebatable

No

Yes

Registration Instructions

VWA, VWR, F8 TO BE CODED

VWR, F8 TO BE CODED

Collection & Request Instructions

2 CITRATE REQUIRED. Usually ordered as part of von Willebrand factor (vWF) studies, which include von Willebrand factor antigen (vWF Ag), FVIII and Ristocetin (RicoF).

If no other tests in vWF studies are ordered, contact request doctor to confirm that no other tests are required.

2 CITRATE REQUIRED. Usually ordered as part of von Willebrand factor (vWF) studies, which include von Willebrand factor antigen (vWF Ag), FVIII, collagen binding factor and Ristocetin (RicoF).

If no other tests in vWF studies are ordered, contact request doctor to confirm that no other tests are required.

Processing Instructions

Centrifuge immediately.

If a tube is collected for vWF RiCoF, this tube needs to be centrifuged twice.

Aliquot all 2 citrate tubes into 2 labelled aliquot tubes and store at -80C.

Centrifuge immediately.

Aliquot all 2 citrate tubes into 2 labelled aliquot tubes and store at -80C.

Registration Instructions

Approval required from the Microbiology Pathologist prior to dispatch

Microbiologist approval is required prior to dispatch.

Test cost $120.