Pathology Handbook

Preferred Container Type

Urine Bottle ACID

Urine Bottle PLAIN

Processing Instructions

Non-acidified urine collections should be mixed thoroughly by inversion x5 immediately prior to acidification (prior to analysis). Acidify to pH < 2.

Weigh, aliquot. Record start and end date/time of collection and volume on bottle/request form.

Weigh, aliquot. Record start and end date/time of collection and volume on bottle/request form.

Processing Instructions

1 mL of citrated plasma required for assay.

Send one aliquot to Monash and freeze the remaining aliquots in -80C.

1 mL of citrated plasma required for assay.

Send one aliquot to Monash and give the other aliquot to the NPV Haematology/Coags scientist to store in the in -80C freezer.

Request Group

056

AJA

Alternative names

Group A Arbovirus PCR

Group A Arbovirus PCR, Ross River Virus PCR, Barmah Forest Virus PCR, Chikungunya Virus PCR, Sindbis Virus PCR

Request Group

TNA

PWT

Laboratory

Haematology

Clinical Biochemistry

Registration Instructions

Turnaround test time approximately 4 weeks. From December 15/12/2021 to 07/01/2022 Specimens are to be frozen and dispatched from the 10/01/2022.

Turnaround test time approximately 4 weeks. From December 19/12/2022 to 06/01/2023 Specimens are to be frozen and dispatched from the 09/01/2023.

Alternative names

Purkinje Cell Antibody, PCA, PCA-1 antibodies, Anti-Yo antibodies, Yo antibodies, Anti-Neuronal Nuclei antibody, Anti-Hu antibodies, Hu antibodies, Anti Ri antibodies, Ri antibodies, Anti CV2 antibodies, CV2 antibodies, Anti Amphiphysin antibodies, Amphiphysin antibodies, Anti-Ma antibodies, Ma antibodies, Anti-Ma1 antibodies, Ma1 antibodies, Anti-Ma2 antibodies, Ma2 antibodies, Anti-PNMA2(Ma2/Ta) Abs, PNMA2 antibodies, Anti SOX1 Abs, SOX1 antibodies, Anti Glia Nuclear antibodies (AGNA), Glia Nuclear antibodies, Paraneoplastic antibodies

Purkinje Cell Antibody, PCA, PCA-1 antibodies, Anti-Yo antibodies, Yo antibodies, Anti-Neuronal Nuclei antibody, Anti-Hu antibodies, Hu antibodies, Anti Ri antibodies, Ri antibodies, Anti CV2 antibodies, CV2 antibodies, Anti Amphiphysin antibodies, Amphiphysin antibodies, Anti-Ma antibodies, Ma antibodies, Anti-Ma1 antibodies, Ma1 antibodies, Anti-Ma2 antibodies, Ma2 antibodies, Anti-PNMA2(Ma2/Ta) Abs, PNMA2 antibodies, Anti SOX1 Abs, SOX1 antibodies, Anti Glia Nuclear antibodies (AGNA), Glia Nuclear antibodies, Paraneoplastic antibodies, GAD 65

Alternative names

BP180, BP230, Collagen VII Elisa, Bullous Pemphigoid

BP180, BP230, Collagen VII Elisa, Bullous Pemphigoid, DSG1, DSG3, Envoplakin

Frequency

Weekly

Fortnightly

Registration Instructions

If this test is requested with combined other allergy tests on the same request, NPV staff are to forward all testing to RCH.

Proceed to testing if it is requested by Dr Ee Lyn Su (immunologist), otherwise the test requires consultant Immunopathologist/Director of Pathology approval.

Specimens will be stored in the Laboratory until the Immunopathologist/Director of Pathology has reviewed the clinical details and/or discussed the request with the requesting clinician.

Cost $50.00

If this test is requested with combined other allergy tests on the same request, NPV staff are to forward all testing to RCH.

Proceed to testing if it is requested by Dr Ee Lyn Su (immunologist) or Dr Brendan McCann (Paediatrician/Allergist), otherwise the test requires consultant Immunopathologist/Director of Pathology approval.

Specimens will be stored in the Laboratory until the Immunopathologist/Director of Pathology has reviewed the clinical details and/or discussed the request with the requesting clinician.

Cost $50.00

Request Group

ICP

ASG

External Laboratory

Institute of Clin.Pathology & Medical Res. (ICPMR)

V.I.D.R.L.

Request Group

MON

SNP

External Laboratory

Monash Pathology

Sullivan Nicolaides Pathology

Request Group

MON

RBW

External Laboratory

Monash Pathology

Royal Brisbane & Womens Hospital

Registration Instructions

CSR pls register as FLW and bring sample to flow department.

Flow scientist to add BCQ

Collect Mon-Thu only.

CSR pls register as FLW and bring sample to flow department.

Flow scientist to add BCQ and re-route sample to Sendaway for Alfred Pathology.

Collect Mon-Thu only.

Request Group

BCQ

FLW

Registration Instructions

Collect Mon-Thu only.

CSR pls register as FLW and bring sample to flow department.

Flow scientist to add BCQ

Collect Mon-Thu only.

Registration Instructions

As NPV is performing concurrent in-house verification, an extra 6ml EDTA tube will be collected. CSR team to pls give the spare sample to either the lab registrar (Matt) or Niki or the flow scientist (Jing).

Collection & Request Instructions

Specimens to be collected only on Monday to Thursday (Except for where a public holiday falls on the following day). Specimens collected after 12pm on Sundays must be stored at room temperature. Specimens more than 24 hours old cannot be tested and a recollection will be requested.

Please collect an extra EDTA tube (using 6mL EDTA) as we are currently performing concurrent in-house verification - please ask for patient permission. CSR team will give the spare sample to either the lab registrar (Matt) or Niki or the flow scientist (Jing).

Please ensure that blood for molecular testing for BCRABL is collected in a separate dedicated sterile tube. Molecular specimens must NOT be put through any analysers. If unsure please call the Genetics and Molecular Pathology laboratory.

Tube handling must be minimized to prevent contamination.

Specimens to be collected only on Monday to Thursday (Except for where a public holiday falls on the following day). Specimens collected after 12pm on Sundays must be stored at room temperature. Specimens more than 24 hours old cannot be tested and a recollection will be requested.

Please ensure that blood for molecular testing for BCRABL is collected in a separate dedicated sterile tube. Molecular specimens must NOT be put through any analysers. If unsure please call the Genetics and Molecular Pathology laboratory.

Tube handling must be minimized to prevent contamination.

Registration Instructions

As NPV is performing concurrent in-house verification, an extra 6ml EDTA tube will be collected. CSR team to pls give the spare sample to either the lab registrar (Matt) or Niki or the flow scientist (Jing).

Collection & Request Instructions

Specimens to be collected only on Monday to Thursday (Except for where a public holiday falls on the following day). Specimens collected after 12pm on Sundays must be stored at room temperature. Specimens more than 24 hours old cannot be tested and a recollection will be requested.

Please ensure that blood for molecular testing for BCRABL is collected in a separate dedicated sterile tube. Molecular specimens must NOT be put through any analysers. If unsure please call the Genetics and Molecular Pathology laboratory.

Tube handling must be minimized to prevent contamination.

Specimens to be collected only on Monday to Thursday (Except for where a public holiday falls on the following day). Specimens collected after 12pm on Sundays must be stored at room temperature. Specimens more than 24 hours old cannot be tested and a recollection will be requested.

Please collect an extra EDTA tube (using 6mL EDTA) as we are currently performing concurrent in-house verification - please ask for patient permission. CSR team will give the spare sample to either the lab registrar (Matt) or Niki or the flow scientist (Jing).

Please ensure that blood for molecular testing for BCRABL is collected in a separate dedicated sterile tube. Molecular specimens must NOT be put through any analysers. If unsure please call the Genetics and Molecular Pathology laboratory.

Tube handling must be minimized to prevent contamination.

Frequency

Weekdays

Alternative names

Peripheral blood smear

Registration Instructions

This test must be ordered by a genetist ( eg. Austin Hospital genetic counselling).Both BRCA 1 and 2 combined.

Cannot be ordered by a GP.

Test costs $800 if not ordered by a Genetist. (Both BRCA 1 and 2 combined)

This test must be ordered by a genetist ( eg. Austin Hospital genetic counselling).Both BRCA 1 and 2 combined.

Cannot be ordered by a GP.

Test costs $800 if not ordered by a Genetist. (Both BRCA 1 and 2 combined)

Specimen viable for testing up to 7 days 

Alternative names

c-KIT

Alternative names

C9

C9, C5b-9

Registration Instructions

Please take dedicated EDTA (purple top) tube. Collect Mon- Thurs only.

This code is not to be used for non-blood specimen (register FAS for non-blood specimen).

Please take dedicated EDTA (purple top) tube. Collect Mon- Thurs only.

This code is not to be used for non-blood specimen (register FAS for non-blood specimen).

As NPV is performing concurrent in-house verification, an extra EDTA tube will be collected. CSR team to pls give the spare sample to either the lab registrar (Matt) or the flow scientist (Jing).

Collection & Request Instructions

Please collect an extra EDTA tube as we are currently performing concurrent in-house verification - please ask for patient permission. CSR team will give the spare sample to either the lab registrar (Matt) or the flow scientist (Jing).

Registration Instructions

Please take dedicated EDTA (purple top) tube. Collect Mon- Thurs only.

This code is not to be used for non-blood specimen.

Please take dedicated EDTA (purple top) tube. Collect Mon- Thurs only.

This code is not to be used for non-blood specimen (register FAS for non-blood specimen).

Request Group

VCS

PAC

Processing Instructions

Store at 4'C

External Transport Instructions

Send at ambient temperature

Send at 4'C with Ice Pack

Frequency

Weekly

No Longer Available

Method

Test Method: IgG, IgA EIA

Reference Ranges:

cutoff value = mean neg control x 2
neg pos > cutoff
equiv +/- 10% cutoff

Units of Measurement: Optical density.

Detects IgG and IgA to C.trachomatis. Specimens for C.trachomatis PCR testing are the preferred method of diagnosis.

Notes

VIDRL Contact: (03) 9342 9647 / (03) 9342 9648

Volume (Adults)

Minimum/Paediatric Volume

Transport Instructions between sites and/or laboratories

___

Assay name

Chlamydia trachomatis Serology (STI/ Genital) (Sendaway Test)

Chlamydia trachomatis Serology (STI/ Genital)

Laboratory

Microbiology

Serology

Request Group

CTS

TNA

Volume (Adults)

6 mL

0.0 mL

Minimum/Paediatric Volume

1 mL

0.0 mL

Preferred Specimen Type

Serum

See Under Collection Instructions

Preferred Container Type

SST

See Under Collection Instructions

Optional Container Type

Plain tube- serum (No Gel)

Transport Instructions between sites and/or laboratories

Room Temperature

External Laboratory

V.I.D.R.L.

External Transport Instructions

Send at ambient temperature

Volume (Adults)

Minimum/Paediatric Volume

Transport Instructions between sites and/or laboratories

___

Request Group

CDI, FPV, FPB

FPB

Request Group

CDI, FPV, FBV

CDI, FPV, FPB

Request Group

CDI

CDI, FPV, FBV

Registration Instructions

Send one aliquot to Alfred and Haematology/Coags scientist to freeze the remaining aliquots in -80C.

Collection & Request Instructions

Specimens cannot be analysed or re- analysed beyond 4 to 6 hours after collection.

Send one aliquot to Alfred and NPV Haematology/Coags scientist to freeze the remaining aliquots in -80C.

Specimens cannot be analysed or re- analysed beyond 4 to 6 hours after collection.

Processing Instructions

Divide the plasma into 2 aliquots. One will be sent to Alfred as per usual and give the other aliquot to the NPV Haematology/Coags scientist  to store in the in -80C freezer.

Registration Instructions

Cost: $50.00

Location: Alfred (ALF), Eastern Health (EAH), Austin (AUP)

Billing: Alfred (ALF), Eastern Health (EAH), Austin (AUP)

Cost: $50.00

Location: Alfred (ALF), Eastern Health (EAH), Austin (AUP), Royal Melbourne Hospital (SMH)

Billing: Alfred (ALF), Eastern Health (EAH), Austin (AUP), Royal Melbourne Hospital (SMH)

Registration Instructions

Cost: $50.00

Cost: $50.00

Location: Alfred (ALF), Eastern Health (EAH), Austin (AUP)

Billing: Alfred (ALF), Eastern Health (EAH), Austin (AUP)

Alternative names

Carbapenem-Resistant Enterobacteriaciae Screen, CRE Culture, CPE

Carbapenem-Resistant Enterobacteriaciae Screen, CRE Culture, CPE, CPO

Assay name

CSF for Flow Cytometry (Sendaway Test)

CSF for Flow Cytometry

Request Group

FAS

FLW

External Laboratory

The Alfred Pathology Service (APS)

External Transport Instructions

Send at 4'C with Ice Pack

Registration Instructions

ALL CSF FLOW CYTOMETRY REQUESTS MUST ALSO HAVE CYTOLOGY REQUESTED.

Samples must be marked “Urgent”. Flow cytometry is NOT available after 1pm on Fridays or during weekends/afterhours. Testing during these times require approval from a laboratory haematologist PRIOR to sample collection. Otherwise, the sample may not be tested in time (within 2-4 hours) or tested at all. Samples not processed in time is non-viable and non-diagnostic.

Please collect a CSF tube with 2-3mL RPMI from Central Specimen Reception BEFORE the CSF sample is collected. CSF should be collected directly into this tube (1 part RPMI; 1 part CSF) to improve sample yield. 

ALL CSF FLOW CYTOMETRY REQUESTS MUST ALSO HAVE CYTOLOGY REQUESTED.

Samples must be marked “Urgent”. Flow cytometry is NOT available after 1pm on Fridays or during weekends/afterhours. Testing during these times require approval from a laboratory haematologist PRIOR to sample collection. Otherwise, the sample may not be tested in time (within 2-4 hours) or tested at all. Samples not processed in time is non-viable and non-diagnostic.

Collection & Request Instructions

Spinal tap should be performed at least 12hrs following suspected SAH.

Send to laboratory Urgently - must be spun within 1hr. Specimen must be transported to the Alfred immediately following microbiological testing. 

Tube 4 is the preferred tube.

Cover sample with foil to protect from light.

Spinal tap should be performed at least 12hrs following suspected SAH.

Send to laboratory Urgently - must be spun within 1hr. Specimen must be transported to the Alfred immediately following microbiological testing. Package specimen in a red specimen bag and attach an urgent sticker to the bag.

Tube 4 is the preferred tube.

Cover sample with foil to protect from light.

External Transport Instructions

Send-away Department:

Send sample urgently to the Alfred. Call Alfred CSR 03 9076 3888 to notify of urgent sample arrival.

Alternative names

Pharmacogenomic Screen, myDNA

Pharmacogenomic Screen, myDNA, CYP2C19, CYP2D6, 2D6, 2C19, 2C9, CYP2C9, CYP3A4, CYP3A5, CYP1A2, VKORC1, SLO1B1

External Transport Instructions

Specimen needs to arrive at St Vincents on the day of collection before 5pm

Bone marrow specimen needs to arrive at St Vincents on the day of collection before 5pm.  Peripheral blood needs to arrive within 24 hours.

External Transport Instructions

Contact numbers:    Specimen Reception 9594 4538

                              Cytogenetics 9594 3471

Request Group

VCS

PAC

External Transport Instructions

Send at ambient temperature

Send at 4'C with Ice Pack

Request Group

DCT

DCT / DAT

MBS rebatable

No

No

Notes

Result Interpretation:
Positive in most Autoimmune Haemolytic Anaemias and post incompatible blood transfusion. Some drugs may cause the result to be positive, e.g. Aldomet.

Result Interpretation:
Positive in most Autoimmune Haemolytic Anaemias and post incompatible blood transfusion. Some drugs may cause the result to be positive, e.g. Aldomet.

Alternative names

Filarial Serology, Filariasis Serology

Filarial Serology, Filariasis Serology, Filaria serology, Filaria antibodies

Registration Instructions

Microbiology pathologist approval required prior to testing. 

Collection & Request Instructions

It is recommended that an FBE and film for microfilaria be performed.

Processing Instructions

Centrifuge
Aliquot

Assay name

Faecal bacterial screen by PCR

Faecal bacterial multiplex PCR

Registration Instructions

Add FPB whenever faeces for micro and culture is requested. This panel complements FMC panel.

Use this panel instead of FPC.

Add FPB whenever faeces for micro and culture is requested. This panel complements FMC panel.

Use this panel instead of FPC.

This assay screens for : Shigella / EIEC, Salmonella, VIbrio, Yersinia, CLostridium difficile toxib B, Aeromonas

Alternative names

shigella, salmonella, vibrio, yersinia, aeromonas, clostridium, faecal multiplex PCR

Alternative names

Bacteria PCR Salmonella screen Shigella screen Campylobacter Vibrio cholerae Vibrio parahaemolyticus

Bacteria PCR Salmonella Shigella Campylobacter Vibrio cholerae Vibrio parahaemolyticus Aeromonas Yersinia Clostridium difficile

Request Group

Molecular test

FPB

Registration Instructions

This test will be discontinued as of 30th August 2016 for patients aged > 4 years. For patients > 4 years the breath hydrogen test is recommended for the investigation of sugar malabsorption.

This test will be discontinued as of 30th August 2016 for patients aged > 4 years. For patients > 4 years the breath hydrogen test is recommended for the investigation of sugar malabsorption.

Assay name

Faecal viral screen by PCR

Faecal viral multiplex PCR

Alternative names

Rotavirus Norovirus

Rotavirus, adenovirus, sapovirus, astrovirus, Norovirus

Registration Instructions

Panel as FPV. This panel replaces ADS, STV, NVP and VIG.

Panel as FPV. This panel replaces ADS, STV, NVP and VIG.

This assay covers norovirus, rotavirus, astrovirus, sapovirus, adenovirus

Request Group

Molecular test

FPV

Request Group

FMC, FPV, FPB

FPB

Request Group

FMC, FPV, FBV

FMC, FPV, FPB

Request Group

FMC

FMC, FPV, FBV

Alternative names

Faecal Parasitic PCR

Faecal Parasitic PCR, Entamoeba histolytica PCR

Registration Instructions

Entamoeba histolytica PCR may be requested on a liver abscess fluid. 

Preferred Specimen Type

Faeces

Faeces
Fluid

Request Group

PWT

FIO

Volume (Adults)

Minimum/Paediatric Volume

Registration Instructions

Could receive 2 x placental, ear, nose or throat swabs

Could receive 2 x placental, ear, nose or throat swabs.

Registration Instructions

FMH - flow cytometry should be sent out during normal business hours only. For samples received after 12pm on Fridays (or day before public holidays), please notify Lab Haematology Registrar or Consultant.

All samples should be sent out with the % & mL estimation by Kleihauer, if available.

Transport Instructions between sites and/or laboratories

Room Temperature
Send to lab immediately

Minimum/Paediatric Volume

0.2 mL

Request Group

KUN

FLA

Registration Instructions

Code FC if blood culture bottles collected.

During work hours please send to microbiology to be split for biochemistry, cytology and other further testing. 

Do not use this panel for joint fluids/aspirates (knee) - JFA

Code FC if blood culture bottles collected.

During work hours please send to microbiology to be split for biochemistry, cytology and other further testing.

Request Group

MON

RBW

External Laboratory

Monash Pathology

Royal Brisbane & Womens Hospital

Request Group

OXF

GAA

Minimum/Paediatric Volume

Volume (Adults)

Minimum/Paediatric Volume

Transport Instructions between sites and/or laboratories

Room Temperature

Frequency

Weekly

Laboratory

Microbiology

Molecular

Collection & Request Instructions

Test only performed on Hepatitis B surface antigen POSITIVE patients unless authorised by Microbiology Registrar.

Test only performed on Hepatitis B surface antigen POSITIVE patients unless authorised by Microbiology Registrar.

If specifically requested to be sent to The Alfred use test code HBL

Assay name

Hepatitis B DNA / Viral Load(Sendaway Test)

Hepatitis B DNA / Viral Load

Request Group

HBL

HBV

Collection & Request Instructions

Test only performed on Hepatitis B surface antigen POSITIVE patients unless authorised by Microbiology Registrar.

Requests for urgent testing must be approved by the Microbiology Unit Head.

Test only performed on Hepatitis B surface antigen POSITIVE patients unless authorised by Microbiology Registrar.

Processing Instructions

Criteria for testing:

Criteria for testing:

External Laboratory

The Alfred Pathology Service (APS)

External Transport Instructions

Send at 4'C with Ice Pack

Frequency

Fortnightly

Daily

Collection & Request Instructions

This test cannot be Bulk Billed if a Hepatitis C PCR is also requested.  

Only 2 tests per 12 month period can be bulk billed once the patient is on treatment.

Requests for urgent testing must be approved by the Microbiology Unit Head.

This test cannot be Bulk Billed if a Hepatitis C PCR is also requested.  

Only 2 tests per 12 month period can be bulk billed once the patient is on treatment.

To be sent to the Alfred only if requested and panel as HVL

Request Group

PMC

SE

Registration Instructions

Sendaway test to:

Peter MacCallum Cancer Centre, Her2 ISH Service

Registration:

Add-on request. Registered into PARIS by Anatomical Pathology

Authorisation:

Pathologist authorisation required.

Pathologist block selection required.

Additional Registration Requirements:

A Peter MacCallum HER2 CISH request form must be completed and accompany the slides.

Order an ADM report: Order an addendum against the biopsy accession. Enter details of the sendaway using ATR (what was sent, to who, address, date sent). Test results are reported into this addendum by the requesting pathologist.

Resulting: Assay results are incorporated into a supplementary report issued by the pathologist.

Send add on request through to Anatomical Pathology at the Alfred via fax (9076 2899) or email anatomicalpathology@alfred.org.au

External Laboratory

Peter MacCallum Cancer Centre (PMCC)

The Alfred Pathology Service (APS)

External Transport Instructions

Address To:

Peter MacCallum Cancer Centre

HER2 ISH Service

Pathology Level 1

St Andrews Place,

East Melbourne VIC 3002

Contact phone: 9656 5815

Contact fax: 9656 1460

Minimum/Paediatric Volume

1 mL

0.2 mL

External Transport Instructions

Send at ambient temperature

Send at 4'C with Ice Pack

Registration Instructions

Test costs $80 as it is a non-rebatable test. Please use the below link to print and complete the patient consent form

https://catalogue.pathology.health.nsw.gov.au/assets/resources/NSWHP%20Payment%20Consent%20Form.pdf

https://www.slhd.nsw.gov.au/SSWPS/Patient_consent_for_non-rebateable_test.pdf

Test costs $80 as it is a non-rebatable test. Please use the below link to print and complete the patient consent form

https://catalogue.pathology.health.nsw.gov.au/assets/resources/NSWHP%20Payment%20Consent%20Form.pdf

Registration Instructions

Test costs $80 as it is a non-rebatable test. Please use the below link to print and complete the patient consent form

https://www.slhd.nsw.gov.au/SSWPS/Patient_consent_for_non-rebateable_test.pdf

Test costs $80 as it is a non-rebatable test. Please use the below link to print and complete the patient consent form

https://catalogue.pathology.health.nsw.gov.au/assets/resources/NSWHP%20Payment%20Consent%20Form.pdf

https://www.slhd.nsw.gov.au/SSWPS/Patient_consent_for_non-rebateable_test.pdf

Alternative names

Antibodies to Infliximab, Remicade, Inflectra

Antibodies to Infliximab, Remicade, Inflectra, IFX

Alternative names

Jak2 V617F

Jak2 V617F, JAK2e14

Request Group

PMC

ALR

MBS rebatable

No

Yes

Registration Instructions

Primary EDTA tube required to be sent to the external laboratory (Not an aliquot)

Only JAK2V617F (or exon 14) can be sent as a single gene request.

JAK2e12 (exon 12) can no longer be requested as a single gene panel. Please refer to the Laboratory Haematology Registrar/Consultant.

Primary EDTA tube required to be sent to the external laboratory (Not an aliquot)

Only JAK2V617F (or exon 14) can be sent as a single gene request. If only "JAK2" is written on the request, please clarify with the Laboratory Haematology Registrar/Consultant.

JAK2e12 (exon 12) can no longer be requested as a single gene panel. Please refer to the Laboratory Haematology Registrar/Consultant.

External Laboratory

Peter MacCallum Cancer Centre

The Alfred Pathology Service (APS)

Collection & Request Instructions

Liase with Haematology Registrar before collection.

Can be stored at 4'C for up to 3 days.

Can be stored at 4'C for up to 3 days.

External Transport Instructions

This requst must be approved by the haematology registrar prior to sending

Registration Instructions

JE and MVE virus infections are urgent notifiable conditions. Clinicians must notify the Department of Health of suspected or confirmed cases immediately by phoning 1300 651 160 (24 hours)

Registration Instructions

JE and MVE virus infections are urgent notifiable conditions. Clinicians must notify the Department of Health of suspected or confirmed cases immediately by phoning 1300 651 160 (24 hours)

Request Group

KUN

FLA

Registration Instructions

Legionella pneumophilia serotype 1 and legionella longbeachae (legionella pneumophilia serotypes 2-7 and legionella micdadei no longer performed as specific reagents no longer available)

Registration Instructions

Specimens will not be accepted for testing between 21/12/2022 and 02/01/2023.

Specimens will not be accepted for testing between 20/12/2022 and 02/01/2023.

Registration Instructions

Specimens will not be accepted for testing between 21/12/2022 and 02/01/2023.

Request Group

FAS

FLW

Registration Instructions

Blood and bone marrow specimens (dedicated EDTA tube) are to be kept at room temperature. 

All other specimens are to be refrigerated at 4^oC - CSF and tissue specimens require RPMI to be added.

CSR to register with generic FLW panel and bring specimen to flow.

Flow scientist: If lymphoma panel is required, add LPD panel. If sendaway for leukaemia and hairy cell leukaemia is required, then add FAS panel and re-route sample and request to Alfred Pathology. (Note: for T-LPD, add PMCC panel instead and re-route sample to PMCC).

Blood and bone marrow specimens (dedicated EDTA tube) are to be kept at room temperature. 

All other specimens are to be refrigerated at 4^oC - CSF and tissue specimens require RPMI to be added.

Alternative names

FACS, Flow cytometry, Lymphocyte Surface Markers, Flow Cytometry, Tissue for Flow Cytometry, CSF for Cytometry, Fluid for Flow Cytometry, Immunophenotyping, Immunophenotype, Peripheral Blood Flow Cytometry, Leucocyte surface markers, Flow (NHL)

FACS, Flow cytometry, Lymphocyte Surface Markers, Flow Cytometry, Tissue for Flow Cytometry, CSF for Cytometry, Fluid for Flow Cytometry, Immunohistochemistry, Immunophenotyping, Immunophenotype, Peripheral Blood Flow Cytometry, Leucocyte surface markers, Flow (NHL)

Collection & Request Instructions

Dedicated EDTA tube required. Please provide relevant clinical history and specify exact type of flow cytometric analysis required.

For peripheral blood sample, an FBE must also be requested. Collect 2xEDTA. 

For CSF sample, please collect a tube with 2-3mL RPMI from Central Specimen Reception BEFORE the CSF sample is collected. CSF should ideally be collected directly into this tube (1 part RPMI 1 part CSF) to improve sample yield. All CSF flow requests must also have CYTOLOGY requested.

Samples must be marked “Urgent”. Flow cytometry is NOT available after 1pm on Fridays or during weekends/afterhours. Testing during these times require approval from a laboratory haematologist PRIOR to sample collection. Otherwise, the sample may not be tested in time (within 2-4 hours for CSF, up to 24 hours for blood sample) or tested at all. Samples not processed in time is non-viable and non-diagnostic. 

Laboratory haematologist can be contacted on 8405 2596 during normal working hours or via switchboard after hours.

Please provide relevant clinical history and specify exact type of flow cytometric analysis required.

For peripheral blood sample, an FBE must also be requested. Collect 2xEDTA. 

For CSF sample, please collect a tube with 2-3mL RPMI from Central Specimen Reception BEFORE the CSF sample is collected. CSF should be collected directly into this tube (1 part RPMI; 1 part CSF) to improve sample yield. All CSF flow requests must also have CYTOLOGY requested.

Samples must be marked “Urgent”. Flow cytometry is NOT available after 1pm on Fridays or during weekends/afterhours. Testing during these times require approval from a laboratory haematologist PRIOR to sample collection. Otherwise, the sample may not be tested in time (within 2-4 hours for CSF, up to 24 hours for blood sample) or tested at all. Samples not processed in time is non-viable and non-diagnostic. 

Laboratory haematologist can be contacted on 8405 2596 during normal working hours or via switchboard after hours.

Registration Instructions

Blood and bone marrow specimens (dedicated EDTA tube) are to be kept at room temperature. 

All other specimens are to be refrigerated at 4^oC - tissue specimens require RPMI to be added.

Blood and bone marrow specimens (dedicated EDTA tube) are to be kept at room temperature. 

All other specimens are to be refrigerated at 4^oC - CSF and tissue specimens require RPMI to be added.

Collection & Request Instructions

A separate container is required for this test.

Collection & Request Instructions

Dedicated EDTA tube required. Please provide relevant clinical history and specify exact type of flow cytometric analysis required.

For peripheral blood sample, an FBE must also be requested. Collect 2xEDTA. 

Samples must be marked “Urgent”. Flow cytometry is NOT available after 1pm on Fridays or during weekends/afterhours. Testing during these times require approval from a laboratory haematologist PRIOR to sample collection. Otherwise, the sample may not be tested in time (within 2-4 hours for CSF, up to 24 hours for blood sample) or tested at all. Samples not processed in time is non-viable and non-diagnostic. All CSF flow requests must also have CYTOLOGY requested.

Laboratory haematologist can be contacted on 8405 2596 during normal working hours or via switchboard after hours.

Dedicated EDTA tube required. Please provide relevant clinical history and specify exact type of flow cytometric analysis required.

For peripheral blood sample, an FBE must also be requested. Collect 2xEDTA. 

For CSF sample, please collect a tube with 2-3mL RPMI from Central Specimen Reception BEFORE the CSF sample is collected. CSF should ideally be collected directly into this tube (1 part RPMI 1 part CSF) to improve sample yield. All CSF flow requests must also have CYTOLOGY requested.

Samples must be marked “Urgent”. Flow cytometry is NOT available after 1pm on Fridays or during weekends/afterhours. Testing during these times require approval from a laboratory haematologist PRIOR to sample collection. Otherwise, the sample may not be tested in time (within 2-4 hours for CSF, up to 24 hours for blood sample) or tested at all. Samples not processed in time is non-viable and non-diagnostic. 

Laboratory haematologist can be contacted on 8405 2596 during normal working hours or via switchboard after hours.

Alternative names

Lupus Inhibitor Screen, LA, LAS

Lupus Inhibitor Screen, LA, LAS, LE

Minimum/Paediatric Volume

0.5 mL

Registration Instructions

For T or B cells alone, use a separate panel code. Testing must be performed within 24 hours of collection.  External laboratory only performs test during weekdays. Performed on patients from 16 years of age and above.  Patients less than 16 years of age testing needs to be referred to RCH.

As NPV is performing concurrent in-house verification, an extra EDTA tube will be collected. CSR team to pls give the spare sample to either the lab registrar (Matt) or Niki or the flow scientist (Jing).

For T or B cells alone, use a separate panel code. Testing must be performed within 24 hours of collection.  External laboratory only performs test during weekdays. Performed on patients from 16 years of age and above.  Patients less than 16 years of age testing needs to be referred to RCH.

Collection & Request Instructions

Please collect an extra EDTA tube as we are currently performing concurrent in-house verification - please ask for patient permission. CSR team will give the spare sample to either the lab registrar (Matt) or the flow scientist (Jing).

Registration Instructions

For T or B cells alone, use a separate panel code. Testing must be performed within 24 hours of collection.  External laboratory only performs test during weekdays. Performed on patients from 16 years of age and above.  Patients less than 16 years of age testing needs to be referred to RCH.

For T or B cells alone, use a separate panel code. Testing must be performed within 24 hours of collection.  External laboratory only performs test during weekdays. Performed on patients from 16 years of age and above.  Patients less than 16 years of age testing needs to be referred to RCH.

As NPV is performing concurrent in-house verification, an extra EDTA tube will be collected. CSR team to pls give the spare sample to either the lab registrar (Matt) or Niki or the flow scientist (Jing).

Collection & Request Instructions

Please collect an extra EDTA tube as we are currently performing concurrent in-house verification - please ask for patient permission. CSR team will give the spare sample to either the lab registrar (Matt) or the flow scientist (Jing).

Assay name

Lymphocyte subset

Lymphocyte subset (Adult)

Registration Instructions

For T or B cells alone, use a separate panel code. Testing must be performed within 24 hours of collection.  External laboratory only performs test during weekdays.

For T or B cells alone, use a separate panel code. Testing must be performed within 24 hours of collection.  External laboratory only performs test during weekdays. Performed on patients from 16 years of age and above.  Patients less than 16 years of age testing needs to be referred to RCH.

Preferred Container Type

Lithium Heparin (without Gel)
EDTA 4 mL

4mL Lithium Heparin and EDTA blood tube

Request Group

RCH

RCH, FBE

Registration Instructions

Collect from Monday to Thursday only. Test must be requested by a Paediatrician (Consultant).  CSR staff check with Haematology Registrar prior to sending.

Collect from Monday to Thursday only. Test must be requested by a Paediatrician (Consultant).  CSR staff check with Haematology Registrar prior to sending.

FBE to performed at NPV and results to be send to RCH with the lithium heparin specimen.

Preferred Container Type

Lithium Heparin (without Gel)

Lithium Heparin (without Gel)
EDTA 4 mL

Optional Container Type

EDTA 4 mL

Request Group

MBC

FLW

Registration Instructions

Dedicated EDTA tube stored at room temperature. Please collect extra EDTA  and  request FBE at same time.

CSR to register as FLW and send sample and request to Flow Department.

Flow scientist to add panel MBC and re-route sample to Sendaway for Alfred Pathology.

Dedicated EDTA tube stored at room temperature. Please collect extra EDTA  and  request FBE at same time.

Registration Instructions

RPMI not necessary however if added will not affect test.

EDTA or Salivary kit are the required samples

Private: $595

Public (Northern Health request form): No charge 

Non-Medicare patients: $510.40 - $589.90

Microarray and Fragile X testing can be performed on one EDTA specimen  

RPMI not necessary however if added will not affect test.

EDTA or Salivary kit are the required samples. For product of conception (POC) see "Cytogentics - Products of Conception"

Private: $595

Public (Northern Health request form): No charge 

Non-Medicare patients: $510.40 - $589.90

Microarray and Fragile X testing can be performed on one EDTA specimen  

Registration Instructions

EDTA or Salivary kit are the required samples

Private: $595

Public (Northern Health request form): No charge 

Non-Medicare patients: $510.40 - $589.90

Microarray and Fragile X testing can be performed on one EDTA specimen  

RPMI not necessary however if added will not affect test.

EDTA or Salivary kit are the required samples

Private: $595

Public (Northern Health request form): No charge 

Non-Medicare patients: $510.40 - $589.90

Microarray and Fragile X testing can be performed on one EDTA specimen  

External Laboratory

V.I.D.R.L.

Request Group

KUN

FLA

Registration Instructions

JE and MVE virus infections are urgent notifiable conditions. Clinicians must notify the Department of Health of suspected or confirmed cases immediately by phoning 1300 651 160 (24 hours)

Registration Instructions

Pathologist authorisation required prior to testing.

The request must specify pan-mycobacterial PCR and there must be an indication on the request whether AFB have been detected by smear microscopy.

Requests that specify AFB PCR, Mycobacterial PCR, or Atypical PCR will not be accepted for testing.

Sterile specimens and tissues will only be processed for this assay  

Alternative names

Anti Myositis antibodies, Anti Mi-2, Mi-2 antibodies, Anti Ku, Ku antibodies, Anti Pm/Scl(75&100), Pm/Scl antibodies, Anti SRP, SRP antibodies, Anti PL7, PL7 antibodies, Anti EJ, EJ antibodies, Anti OJ, OJ antibodies, Anti Ro-52, R0-52 antibodies, TIF1Y(P155), Anti synthetase

Anti Myositis antibodies, Anti Mi-2, Mi-2 antibodies, Anti Ku, Ku antibodies, Anti Pm/Scl(75&100), Pm/Scl antibodies, Anti SRP, SRP antibodies, Anti PL7, PL7 antibodies, Anti EJ, EJ antibodies, Anti OJ, OJ antibodies, Anti Ro-52, R0-52 antibodies, TIF1Y(P155), Anti synthetase, MDA5

Alternative names

NTBNP, Pro-BNP

NTBNP, Pro-BNP, Brain natriuretic peptide

Registration Instructions

Doctor : TNHSH

Bill to : INFOE

Tests included:

Anti-Hepatitis B Surface Antibody

Doctor : NHIC

Bill to : INFOE

Tests included:

Anti-Hepatitis B Surface Antibody

Registration Instructions

Tests included:

Anti-Hepatitis B Surface Antibody

Doctor : TNHSH

Bill to : INFOE

Tests included:

Anti-Hepatitis B Surface Antibody

Registration Instructions

Doctor : TNHSH

Bill to : INFOE

Tests included:

HIV Antigen/Antibody Combo

Anti-Hepatitis C Antibody

Hepatitis B Surface Antigen

Doctor : NHIC

Bill to : INFOE

Tests included:

HIV Antigen/Antibody Combo

Anti-Hepatitis C Antibody

Hepatitis B Surface Antigen

Registration Instructions

Tests included:

HIV Antigen/Antibody Combo

Anti-Hepatitis C Antibody

Hepatitis B Surface Antigen

Doctor : TNHSH

Bill to : INFOE

Tests included:

HIV Antigen/Antibody Combo

Anti-Hepatitis C Antibody

Hepatitis B Surface Antigen

Collection & Request Instructions

Patient to book appointment directly with Alfred Pathology by calling 9076 3109

Test not available on Mondays and Fridays

Testing needs to be completed within 4 hours of collection

Test also available at Austin Pathology Monday to Friday between 9am and midday. To book, phone 9496 5472. Please also refer to Austin Pathology website for details. https://www.austinpathology.org.au/test-directory/1351

Patient to book appointment directly with Alfred Pathology by calling 9076 3109

Test not available on Mondays and Fridays

Testing needs to be completed within 6 hours of collection

Test also available at Austin Pathology Monday to Friday between 9am and midday. To book, phone 9496 5472. Please also refer to Austin Pathology website for details. https://www.austinpathology.org.au/test-directory/1351

Frequency

Weekdays

Preferred Container Type

SST

SST (collect on ice)

Alternative names

sFlt-1, PLGF, PET

sFlt-1, PLGF, PET, PERT

Frequency

Weekly

Fortnightly

Frequency

Weekly

Fortnightly

Request Group

QFS

ARR

Collection & Request Instructions

Routine testing detects both Phase I (chronic Q fever) and Phase II antibodies (acute Q fever).

External Laboratory

V.I.D.R.L.

Australian Rickettsial Reference Laboratory

Alternative names

Renal Antibody Titration

Renal Antibody Titration, Isohaemagglutinin

Request Group

MISC S

TNA

Volume (Adults)

2 mL

0.0 mL

Preferred Specimen Type

Serum

See Under Collection Instructions

Preferred Container Type

SST

See Under Collection Instructions

Transport Instructions between sites and/or laboratories

Room Temperature

___

External Laboratory

Institute of Clin.Pathology & Medical Res. (ICPMR)

External Transport Instructions

Send at 4'C with Ice Pack

Frequency

As Required

No Longer Available

Collection & Request Instructions

CSF specimens for syphilis serology must be accompanied by a serum specimen. CSF specimens will only be tested if the patient's serum tests positive for syphilis.

CSF specimens will only be tested if the patient's serum tests positive for syphilis.

Request Group

PAH

AUP

External Laboratory

Princess Alexandra Hospital

Austin Pathology

External Transport Instructions

Specimens are to be shipped to the following address:

Pathology Queensland,

Central Specimen Reception,

Level 3, Block 7,

Royal Brisbane Hospital

Herston, Queensland 4029

Request Group

THL, FBE, IS

THL, FBE, FER

Request Group

MON, THL, FBE, IS

THL, FBE, IS

Registration Instructions

2 x EDTA lavender top tube required for thalassaemia testing (1x Hb Electrophoresis, 1x DNA testing if required)

1 x Serum gold top tube

Please register THL, FBE and IS panel when for all thalassaemia screens.

If the word gene or DNA is used in the request, this is for genetic test. Thalassaemia genetic test is performed at Monash, register as MON. ALL Thalassaemia DNA analysis must be approved by the Haematology Scientist, Registrar or Consultant first. DO NOT SEND TO MONASH DIRECTLY FROM CSR. All samples requiring DNA analysis will be packaged up by the scientist prior to handing over to the sendaway department for sendout. 

FBE, iron studies and Hb electrophoresis results must be sent together with second EDTA tube.

All samples requiring DNA analysis will be packaged up by the scientist prior ro handing over to the sendaway department for sendout.

ONLY use this code if the word gene or DNA is used in the request. Otherwise use HBF (haemoglobin electrophoresis).

Patient needs to provided Australian Medicare number and Victoria address for state goverment grant (No charge). For Overseas residents with Victorian address a bill will be issued.

FBE, ferritin and heamoglobin electrophoresis results are required to be sent to Monash to finalise the result.

2 x EDTA lavender top tube required for thalassaemia testing (1x Hb Electrophoresis, 1x DNA testing if required)

1 x Serum gold top tube

Please register THL, FBE and IS panel when for all thalassaemia screens.

DO NOT SEND TO MONASH DIRECTLY FROM CSR.  If the word gene or DNA is used in the request, this is for genetic test. Thalassaemia genetic test is performed at Monash, register as MON. ALL Thalassaemia DNA analysis must be approved by the Haematology Scientist, Registrar or Consultant first.  All samples requiring DNA analysis will be packaged up by the scientist prior to handing over to the sendaway department for sendout. 

FBE, iron studies and Hb electrophoresis results must be sent together with second EDTA tube.

All samples requiring DNA analysis will be packaged up by the scientist prior ro handing over to the sendaway department for sendout.

ONLY use this code if the word gene or DNA is used in the request. Otherwise use HBF (haemoglobin electrophoresis).

Patient needs to provided Australian Medicare number and Victoria address for state goverment grant (No charge). For Overseas residents with Victorian address a bill will be issued.

FBE, ferritin and heamoglobin electrophoresis results are required to be sent to Monash to finalise the result.

Registration Instructions

2x EDTA lavender top tube required for thalassaemia testing (1x Hb Electophoresis, 1x DNA testing if required)

1x Serum gold top tube

Please register THL, FBE and FER panel when for all thalassaemia screens.

If the word gene or DNA is used in the request, this is for genetic test. Thalassaemia genetic test is performed at Monash, register as MON. ALL Thalassaemia DNA analysis must be approved by the Haematology Scientist, Registrar or Consultant first. DO NOT SEND TO MONASH DIRECTLY FROM CSR. All samples requiring DNA analysis will be packaged up by the scientist prior to handing over to the sendaway department for sendout. 

FBE, ferritin and Hb electrophoresis results must be sent together with second EDTA tube.

2x EDTA lavender top tube required for thalassaemia testing (1x Hb Electophoresis, 1x DNA testing if required)

1x Serum gold top tube

Please register THL, FBE and FER panel when for all thalassaemia screens.

DO NOT SEND TO MONASH DIRECTLY FROM CSR. If the word gene or DNA is used in the request, this is for genetic test. Thalassaemia genetic test is performed at Monash, register as MON. ALL Thalassaemia DNA analysis must be approved by the Haematology Scientist, Registrar or Consultant first. DO NOT SEND TO MONASH DIRECTLY FROM CSR. All samples requiring DNA analysis will be packaged up by the scientist prior to handing over to the sendaway department for sendout. 

FBE, ferritin and Hb electrophoresis results must be sent together with second EDTA tube.

Alternative names

Urine casts

Request Group

UM

UMC

Registration Instructions

For Schistosomiasis haematobium in urine, collection of a whole void specimen taken anytime between 10:00 and 14:00 in a urine container withour preservatives is recommended. Peak egg excretion is between 10:00 and 14:00

CSR staff please make a photocopy of the request form for microbiology. 

For Schistosomiasis haematobium in urine, collection of a whole void specimen taken anytime between 10:00 and 14:00 in a urine container withour preservatives is recommended. Peak egg excretion is between 10:00 and 14:00

Alternative names

Ebola virus, Marburg virus, Lassa virus, Crimean - Congo virus haemorrhagic fever

Ebola virus, Marburg virus, Lassa virus, Crimean - Congo virus haemorrhagic fever, Rift valley

Volume (Adults)

Minimum/Paediatric Volume

Transport Instructions between sites and/or laboratories

See Detailed Information

Frequency

Fortnightly

Weekly

Laboratory

Microbiology

Molecular