Pathology Handbook

New search

Fungal PCR (Sendaway Test)

Alternative Names:

Pan Fungal PCR

Laboratory:

Microbiology

Test Code:

FUP

Specimen Types:

Tissue
Bronchoalveolar Lavage
Bronchial Washings
Cerebrospinal Fluid

Container Types:

Plain 70mL pot
CSF Tube 3

Adults Volume or Mass:

1 mL

Collection & Request Instructions:

Department head authorisation required. (ID consult team exempt).

Transport Instructions:

Room Temperature

Check Fungal culture result PRIOR to sending sample.

DO NOT send if culture positive for filamentous fungi or yeast.

Processing Instructions:

PCR can be used to detect fungal DNA in a variety of clinical specimens including tissue, CSF, BAL and vitreous fluid. ICPMR does not perform Panfungal PCR on sputum and blood samples.

PCR can be used to identify fungi from pure culture. It is particularly helpful to identify risk group 3 pathogens, slow growing or poorly sporulating moulds.

Sample requirements:

Fresh tissue or 10x10um paraffin embedded tissue sections in a sterile container, 0.5 mL CSF, 1 mL BAL, 0.2 mL vitreous fluid.

Culture: pure fungal culture

Assay Frequency:

Fortnightly

External Laboratory:

Institute of Clin.Pathology & Medical Res. (ICPMR)

External Transport Instructions:

Send at ambient temperature