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PLEASE NOTE: Recent changes have been made to this Test

Recent changes for Leucocyte Immunophenotyping (Sendaway Test)

Recent changes for Leucocyte Immunophenotyping (Sendaway Test)
Date	Field	Changed From	Changed To
9th April 2024	Registration Instructions	CSR to register with generic FLW panel and bring specimen to flow. Flow scientist: If lymphoma panel is required, add LPD panel. If sendaway for leukaemia is required, then add FAS panel and re-route sample and request to Alfred Pathology. (Note: for paediatric leukaemia, add RCH panel and re-route sample and request to Royal Children's Hospital). Blood and bone marrow specimens (dedicated EDTA tube) are to be kept at room temperature. All other specimens are to be refrigerated at 4^oC - CSF and tissue specimens require RPMI to be added.	CSR to register with generic FLW panel and bring specimen to flow. Flow scientist: If lymphoma/leukaemia panel is required, add LPD panel. If sendaway for external leukaemia panel is required, then add FAS panel and re-route sample and request to Alfred Pathology. (Note: for paediatric leukaemia, add RCH panel and re-route sample and request to Royal Children's Hospital). Blood and bone marrow specimens (dedicated EDTA tube) are to be kept at room temperature. All other specimens are to be refrigerated at 4^oC - CSF and tissue specimens require RPMI to be added.
9th April 2024	Registration Instructions	CSR to register with generic FLW panel and bring specimen to flow. Flow scientist: If lymphoma panel is required, add LPD panel. If sendaway for leukaemia and hairy cell leukaemia is required, then add FAS panel and re-route sample and request to Alfred Pathology. (Note: for T-LPD, add PMCC panel instead and re-route sample to PMCC). Blood and bone marrow specimens (dedicated EDTA tube) are to be kept at room temperature. All other specimens are to be refrigerated at 4^oC - CSF and tissue specimens require RPMI to be added.	CSR to register with generic FLW panel and bring specimen to flow. Flow scientist: If lymphoma panel is required, add LPD panel. If sendaway for leukaemia is required, then add FAS panel and re-route sample and request to Alfred Pathology. (Note: for paediatric leukaemia, add RCH panel and re-route sample and request to Royal Children's Hospital). Blood and bone marrow specimens (dedicated EDTA tube) are to be kept at room temperature. All other specimens are to be refrigerated at 4^oC - CSF and tissue specimens require RPMI to be added.

Date

Field

Changed From

Changed To

9th April 2024

Registration Instructions

CSR to register with generic FLW panel and bring specimen to flow.

Flow scientist: If lymphoma panel is required, add LPD panel. If sendaway for leukaemia is required, then add FAS panel and re-route sample and request to Alfred Pathology. (Note: for paediatric leukaemia, add RCH panel and re-route sample and request to Royal Children's Hospital).

Blood and bone marrow specimens (dedicated EDTA tube) are to be kept at room temperature.

All other specimens are to be refrigerated at 4^oC - CSF and tissue specimens require RPMI to be added.

CSR to register with generic FLW panel and bring specimen to flow.

Flow scientist: If lymphoma/leukaemia panel is required, add LPD panel. If sendaway for external leukaemia panel is required, then add FAS panel and re-route sample and request to Alfred Pathology. (Note: for paediatric leukaemia, add RCH panel and re-route sample and request to Royal Children's Hospital).

Blood and bone marrow specimens (dedicated EDTA tube) are to be kept at room temperature.

All other specimens are to be refrigerated at 4^oC - CSF and tissue specimens require RPMI to be added.

9th April 2024

Registration Instructions

CSR to register with generic FLW panel and bring specimen to flow.

Flow scientist: If lymphoma panel is required, add LPD panel. If sendaway for leukaemia and hairy cell leukaemia is required, then add FAS panel and re-route sample and request to Alfred Pathology. (Note: for T-LPD, add PMCC panel instead and re-route sample to PMCC).

Blood and bone marrow specimens (dedicated EDTA tube) are to be kept at room temperature.

All other specimens are to be refrigerated at 4^oC - CSF and tissue specimens require RPMI to be added.

CSR to register with generic FLW panel and bring specimen to flow.

Blood and bone marrow specimens (dedicated EDTA tube) are to be kept at room temperature.

All other specimens are to be refrigerated at 4^oC - CSF and tissue specimens require RPMI to be added.

Leucocyte Immunophenotyping (Sendaway Test)

Alternative Names:

FACS
Flow cytometry
Lymphocyte Surface Markers
Flow Cytometry
Tissue for Flow Cytometry
CSF for Cytometry
Fluid for Flow Cytometry
Immunohistochemistry
Immunophenotyping
Immunophenotype
Peripheral Blood Flow Cytometry
Leucocyte surface markers
Flow (NHL)

Laboratory:

Haematology

Test Code:

FLW

Registration Instructions:

CSR to register with generic FLW panel and bring specimen to flow.

Blood and bone marrow specimens (dedicated EDTA tube) are to be kept at room temperature.

All other specimens are to be refrigerated at 4^oC - CSF and tissue specimens require RPMI to be added.

Specimen Types:

Blood
Bone Marrow
Tissue
Cerebrospinal Fluid
Fluid
Bronchial Washings
Bronchoalveolar Lavage

Container Types:

EDTA 3 mL
Fresh Tissue Pot
CSF Tube 3
Lithium Heparin (with Gel)

Adults Volume or Mass:

3 mL

Collection & Request Instructions:

Please provide relevant clinical history and specify exact type of flow cytometric analysis required.

For peripheral blood sample, an FBE must also be requested. Collect 2xEDTA.

For CSF sample, please collect a tube with 2-3mL RPMI from Central Specimen Reception BEFORE the CSF sample is collected. CSF should be collected directly into this tube (1 part RPMI; 1 part CSF) to improve sample yield. All CSF flow requests must also have CYTOLOGY requested.

Samples must be marked “Urgent”. Flow cytometry is NOT available after 1pm on Fridays or during weekends/afterhours. Testing during these times require approval from a laboratory haematologist PRIOR to sample collection. Otherwise, the sample may not be tested in time (within 2-4 hours for CSF, up to 24 hours for blood sample) or tested at all. Samples not processed in time is non-viable and non-diagnostic.

Laboratory haematologist can be contacted on 8405 2596 during normal working hours or via switchboard after hours.

Transport Instructions:

Send to lab immediately

Processing Instructions:

Do Not Centrifuge

Assay Frequency:

Weekdays

External Laboratory:

The Alfred Pathology Service (APS)

External Transport Instructions:

Send at ambient temperature