The blood collection tubes supplied are for use only with the QuantiFERON®-TB system (QFT®). The following instructions relate solely to the use of QFT® blood collection tubes.
Antigens have been dried onto the inner wall of the blood collection tubes so it is essential that the contents of the tubes be thoroughly mixed with the blood. The tubes must be transferred to a 37oC incubator as soon as possible and within 16 hours of collection.
The following procedures should be followed for optimal results:
Blood Collection:
For each subject collect 1mL of blood by venipuncture directly into each of the QFT® blood collection tubes.
Order of draw:
1. Grey (nil) tube
2. Green (TB1 antigen) tube
3. Yellow (TB2 antigen) tube
4. Purple (mitogen) tube·
As 1mL tubes draw blood relatively slowly, keep the tube on the needle for 2-3 seconds once the tube appears to have completed filling, to ensure that the correct volume is drawn.
The black mark on the side of the tubes indicates the 1mL fill volume. QFT® blood collection tubes have been validated for volumes ranging from 0.8 to 1.2 mL. If the level of blood in any tube is not close to the indicator line, it is recommended to obtain another blood sample.
If a “butterfly needle” is used, prime tubing with a “purge” tube before filling the QFT® tubes.
Mix by vigorously shaking the tubes up and down 10 times to ensure that the entire inner surface of the tube has been coated with the blood.
Thorough mixing is required to ensure complete integration of the tube’s contents into the blood.
Label tubes appropriately. |