Pathology Handbook

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PLEASE NOTE: Recent changes have been made to this Test


 Recent changes for Cytology - Oesophageal, Bile Duct, Pancreatic & Ampulla Specimens

Recent changes for Cytology - Oesophageal, Bile Duct, Pancreatic & Ampulla Specimens
DateFieldChanged FromChanged To
11th November 2024
Preferred Container Type

Plain 70mL pot
Slide Cytomailer

Slide Cytomailer
Plain 70mL pot
Formalin Pot

11th November 2024
Registration Instructions

Please provide a completed pathology request form (Paper or EMR), including relevant clinical information/patient history.

Cytology - Oesophageal, Bile Duct, Pancreatic & Ampulla Specimens

Alternative Names:Oesophageal Fluid for Cytology
Bile Duct Fluid for Cytology
Pancreatic Fluid for Cytology
Ampulla Fluid for Cytology
Laboratory:Anatomical Pathology
Test Code:CY
Specimen Types:Fluid
Smear
Container Types:
Slide Cytomailer
Plain 70mL pot
Formalin Pot
Plain 70mL pot
Collection & Request Instructions:

Oesophageal, Common Bile Duct , Pancreatic and Ampulla brushings and fluids for Cytology

Please note: Specimen/s can be shared with Microbiology, Biochemistry and Flow Cytometry. Please check other laboratory collection requirements.

Please check other laboratory collection requirements. 

Smear/Slide Preparation: Label glass slide in pencil on the frosted end with the patients full name, date of birth, UR number and the specimen site of collection. 

When the lesion has been brushed, immediately, gently and rapidly rotate the brush on the surface of the prepared slide. Care should be exercised not to press too hard,in order to prevent crushing of the cells. The cellular material should occupy the central portion of the slide. After the slides are prepared, immediately spray the entire slide (distance of approximately 20cm) with spray fixative (cytology fixative/95% alcohol). Rapid and thorough fixation is crucial.

Allow the slide to dry.

Place the slide into a Cytomailer and secure closed.

Place the Cytomailer(s) into a biohazards bag for safe transportation.

Detach the brush tip and place into labeled Formalin Pot. Place into a separate biohazard bag for safe transportation.

Fluid collection: Clearly label the collection container (70ml pot) with patient’s full name, DOB, UR number, date, time and site of collection.

Collect fluid in labelled pot, and tightly close the lid to avoid spillage/leaking. 

Place the specimen pot into a biohazard bag for safe transportation.

 

Transport Instructions:Room Temperature

Please ensure Cytology specimens are sent in a separate Biohazard bag from any accompanying Histology biopsy specimens (Formalin pots). Formalin vapour affects cytology specimen preservation.

Assay Frequency:Weekdays