Pathology Handbook

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Cytogenetics - Solid Tumour (Sendaway Test)

Alternative Names:

Chromosome Analysis - Solid Tumour
Karyotype Analysis - Solid Tumour
Histology-Cytogenetics Solid Tumour

Laboratory:

Anatomical Pathology

Test Code:

CGT

Registration Instructions:

Sendaway test to: Victorian Cancer Cytogenetics Service, St Vincent’s Hospital Melbourne.

Registration: Sendaway registration of solid tumour specimens is performed by Anatomical Pathology.

Authorisation: Pathologist authorisation required. Sample selection requires pathologist triage.

Specimen Types:

Tissue
Paraffin Section (unstained)

Container Types:

Formalin Pot
Fresh Tissue Pot

Collection & Request Instructions:

Conventional cytogentics analysis of solid tumours using cultured fresh tissue is no longer available through VCCS (26/6/13). Fluorescence in situ hybridization (FISH) testing on paraffin embedded tissue sections has obviated the need for conventional cytogenetic analysis.

Specimens can be sent fresh or in formalin.

Specimen triage will be performed in Anatomical Pathology by an APS pathologist.

Transport Instructions:

Room Temperature

Processing Instructions:

http://www.vcgs.org.au/pathology/sections/Metaboli

Paraffin Section preparation for FISH

Requirements:

Cut x1 HE of pathologist selected block (slide should be marked to indicate optimal region for FISH)

Cut x6 slides at 2 micron onto positively charged slides. Note: Section thickness of 2 micron is essential.

Copy of request slip with preferred FISH probe indicated

Assay Frequency:

Weekly

External Laboratory:

St Vincent's Pathology

External Transport Instructions:

Send at ambient temperature

Address to:

Victorian Cancer Cytogenetics Service,

St Vincents Pathology

Level 2, Inpatient Services Building

41 Victoria Parade, Fitzroy

Contact Number for Cytogenetics: 9288 4154