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PLEASE NOTE: Recent changes have been made to this Test


 Recent changes for Acanthamoeba Culture

Recent changes for Acanthamoeba Culture
DateFieldChanged FromChanged To
12th February 2024
Collection & Request Instructions

Scraping MUST be placed directly on Non Nutrient Agar slope available from Microbiology Laboratory.

A slide should also be inoculated for microscopic examination.  This is in addition to the slide inoculated for bacterial investigation.

Collection after 12 noon on Friday requires prior approval by Medical Microbiologist.

A deep collection of corneal tissue is optimal. Swabs are inadequate and collection of corneal material with swabs should be avoided.

Acceptable specimens are:

corneal biopsies

corneal scrapings

contact lenses and contents of contact lens case

contact lens solution - by request only

Note, as this test is not covered by Medicare, non-Northern Health patients will be responsible for the cost incurred, and patient consent must be obtained.    As at July 2023, this fee is $50.

Scraping MUST be placed directly on Non Nutrient Agar slope available from Microbiology Laboratory.

A slide should also be inoculated for microscopic examination.  This is in addition to the slide inoculated for bacterial investigation.

Collection after 12 noon on Friday requires prior approval by Medical Microbiologist.

A deep collection of corneal tissue is optimal. Swabs are inadequate and collection of corneal material with swabs should be avoided.

Acceptable specimens are:

corneal biopsies

corneal scrapings

contact lenses and contents of contact lens case

contact lens solution - by request only

Note, as this test is not covered by Medicare, non-Northern Health patients will be responsible for the cost incurred, and patient consent must be obtained.   

7th February 2024
Collection & Request Instructions

Scraping MUST be placed directly on Non Nutrient Agar slope available from Microbiology Laboratory.

A slide should also be inoculated for microscopic examination.  This is in addition to the slide inoculated for bacterial investigation.

Collection after 12 noon on Friday requires prior approval by Medical Microbiologist.

A deep collection of corneal tissue is optimal. Swabs are inadequate and collection of corneal material with swabs should be avoided.

Acceptable specimens are:

corneal biopsies

corneal scrapings

contact lenses and contents of contact lens case

contact lens solution - by request only

 

Scraping MUST be placed directly on Non Nutrient Agar slope available from Microbiology Laboratory.

A slide should also be inoculated for microscopic examination.  This is in addition to the slide inoculated for bacterial investigation.

Collection after 12 noon on Friday requires prior approval by Medical Microbiologist.

A deep collection of corneal tissue is optimal. Swabs are inadequate and collection of corneal material with swabs should be avoided.

Acceptable specimens are:

corneal biopsies

corneal scrapings

contact lenses and contents of contact lens case

contact lens solution - by request only

Note, as this test is not covered by Medicare, non-Northern Health patients will be responsible for the cost incurred, and patient consent must be obtained.    As at July 2023, this fee is $50.

Acanthamoeba Culture

Laboratory:Microbiology
Test Code:ACN
Specimen Types:Corneal Scraping
Cerebrospinal Fluid
Container Types:
See Under Collection Instructions
Slide
CSF Tube 3
Collection & Request Instructions:

Scraping MUST be placed directly on Non Nutrient Agar slope available from Microbiology Laboratory.

A slide should also be inoculated for microscopic examination.  This is in addition to the slide inoculated for bacterial investigation.

Collection after 12 noon on Friday requires prior approval by Medical Microbiologist.

A deep collection of corneal tissue is optimal. Swabs are inadequate and collection of corneal material with swabs should be avoided.

Acceptable specimens are:

corneal biopsies

corneal scrapings

contact lenses and contents of contact lens case

contact lens solution - by request only

Note, as this test is not covered by Medicare, non-Northern Health patients will be responsible for the cost incurred, and patient consent must be obtained.   

Transport Instructions:Room Temperature
Processing Instructions:http://www.vcgs.org.au/pathology/sections/Metaboli

Non nutrient agar slopes are available in the Microbiology walk in fridge.

Store NNA slopes at room temperature overnight and dispatch to St Vincent's Pathology the following morning. Do not refrigerate.

Specimens arriving after 5pm on a Friday MUST be sent by courier Saturday morning.  Leave a note for the Saturday Set-Up person to pack specimen and arrange a courier via CSP.

Send within 24 hours of collection.

Assay Frequency:Weekdays
External Laboratory:St Vincent's Pathology
External Transport Instructions:Send at ambient temperature

 

To pack specimens Saturday morning:

Log in the specimen and parafilm the lid of the slope. Generate labels.

Refer to instructions in the Serology Sendaway Manual (Document "CD_MI_0369_Serology Sendaway Procedure") to generate an external referral form and pack the specimen.

Use the transport label in the "St Vincent's Pathology" file in Serology to label the outside of the box.

Attach aliquot label to Serology Sendaway Checklist and initial as a record of the specimen being sent.

Deliver the box to CSP and ask them to arrange a courier for an urgent specimen.